| Detailed Information for Phage Espica |
| Discovery Information |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Leah Roach |
| Year Found | 2014 |
| Location Found | Fort Myers, FL USA |
| Finding Institution | Florida Gulf Coast University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 26.419272 N, 81.807501 W Map |
| Discovery Notes | The phage was isolated from a feces sample pulled from a manure pile at a horse far. There were six horses and a mule contributing to this pile, one of the horses was ill. It is unclear whether the sample comes from the sick or the healthy horses.The sample was initially pulled by Cassandra Ellis. |
| Naming Notes | I chose the name in part for the binary star system Spica and then added the E to the front because I like the way it sounds. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 5, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2649 |
| Genome length (bp) | 46537 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGTGAT |
| End Determination Notes | Buildups of reads in Illumina shotgun sequencing. |
| GC Content | 58.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Alliance University (formerly Nyack College) |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque diameter was consistently approximately 1mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 68 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK524487 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
124 |
| Pitt Freezer Box Grid# |
C5 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
