| Detailed Information for Phage Eyes | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Madison Hanaway |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | -8°C |
| GPS Coordinates | 40.438889 N, 79.954722 W Map |
| Discovery Notes | The ground was frozen solid. The soil sample was taken from the very surface layer of the ground next to a metal fence. |
| Naming Notes | Phage Morphology looks like a cloudy iris with a clear pupil. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 18, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1539 |
| Genome length (bp) | 46772 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGCCGCGGTA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DB |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Clear center with large cloudy area on the outside |
| Morphotype | Siphoviridae |
| Number of Genes | 65 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
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| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK061410 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.33x10^11 pfu/mL |
| Date of SEA Lysate Titering | Feb 22, 2017 |
| Pitt Freezer Box# | 55 |
| Pitt Freezer Box Grid# | F7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
