| Detailed Information for Phage Gaia |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Benjamin Yoder |
| Year Found | 2013 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.446389 N, 79.9575 W Map |
| Naming Notes | From the Greek being that represented mother earth. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 13, 2013 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 3247 |
| Genome length (bp) | 90460 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCAGCTG |
| GC Content | 56.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | X |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Lysogeny Notes | This phage has only made leaky lysogens so far. |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | The mycobacteriophage Gaia forms small clear plaques at 30 degrees Celsius. However at 37 degrees Celsius the plaques become a little smaller and less numerous. At 37 the plaques also become cloudy instead of perfectly clear. |
| Morphotype | Siphoviridae |
| Number of Genes | 179 |
| Number of tRNAs | 4 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KJ567043 |
| Refseq Number | NC_026590 |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
1 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |