The Actinobacteriophage Database

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Detailed Information for Phage Gingerbug
Discovery Information
Isolation Host	Microbacterium foliorum NRRL B-24224
Found By	Hope Poet
Year Found	2021
Location Found	Newport, OR United States
Finding Institution	Oregon Coast Community College
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	44.60271 N, 124.04604 W Map
Discovery Notes	I found my soil sample on 10/19/21 at 11:50 am from a flower bed located on the grounds of the college. The soil was very damp and of good quality.
Naming Notes	I named mine gingerbug to represent a piece of me. I have bright red hair and people like to call me ginger!
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 17, 2022
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	1343
Genome length (bp)	39721
Character of genome ends	Circularly Permuted
GC Content	69.3%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	GF
Subcluster	--
Cluster Life Cycle	Unknown
Other Cluster Members	Click to View DelaGarza Gingerbug Lesiram Teng Wrackline
Annotating Institution	Oregon Coast Community College
Annotation Status	In GenBank
Plaque Notes	Plaque morphology consists of small and medium-sized clearings with a few cloudy ones.
Number of Genes	69
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Gingerbug_1 (helix-turn-helix DNA binding domain protein) Gingerbug_2 Gingerbug_3 Gingerbug_4 Gingerbug_5 (terminase) Gingerbug_6 (portal protein) Gingerbug_7 (minor capsid protein) Gingerbug_8 (scaffolding protein) Gingerbug_9 (major capsid protein) Gingerbug_10 Gingerbug_11 (head-to-tail adaptor) Gingerbug_12 (head-to-tail stopper) Gingerbug_13 (minor capsid protein) Gingerbug_14 (tail terminator) Gingerbug_15 (major tail protein) Gingerbug_16 (tail assembly chaperone) Gingerbug_17 (tail assembly chaperone) Gingerbug_18 (tape measure protein) Gingerbug_19 (minor tail protein) Gingerbug_20 (minor tail protein) Gingerbug_21 Gingerbug_22 (minor tail protein) Gingerbug_23 (minor tail protein) Gingerbug_24 Gingerbug_25 Gingerbug_26 (endolysin) Gingerbug_27 Gingerbug_28 Gingerbug_29 (holin) Gingerbug_30 Gingerbug_31 (HNH endonuclease) Gingerbug_32 Gingerbug_33 Gingerbug_34 Gingerbug_35 (winged helix-turn-helix DNA binding domain protein) Gingerbug_36 Gingerbug_37 Gingerbug_38 Gingerbug_39 Gingerbug_40 (helix-turn-helix DNA binding domain protein) Gingerbug_41 (RusA-like resolvase) Gingerbug_42 (DNA binding protein) Gingerbug_43 (helicase loader) Gingerbug_44 (helix-turn-helix DNA binding domain protein) Gingerbug_45 Gingerbug_46 Gingerbug_47 Gingerbug_48 Gingerbug_49 (exonuclease) Gingerbug_50 (ssDNA binding protein) Gingerbug_51 Gingerbug_52 Gingerbug_53 Gingerbug_54 Gingerbug_55 Gingerbug_56 (DNA polymerase III sliding clamp beta) Gingerbug_57 (HNH endonuclease) Gingerbug_58 Gingerbug_59 Gingerbug_60 (ParB-like nuclease domain protein) Gingerbug_61 Gingerbug_62 Gingerbug_63 Gingerbug_64 Gingerbug_65 Gingerbug_66 Gingerbug_67 Gingerbug_68 Gingerbug_69
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	ON970592
Refseq Number	None yet
Published in Paper	Fisher et al 2022, Microbiology Resource Announcements: Complete Annotated Genome Sequences of Two Novel Microbacteriophages, Gingerbug and HerculesXL, from Western Oregon, USA
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	143
Pitt Freezer Box Grid#	A12
Available Files
Plaque Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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