| Detailed Information for Phage Wrackline | |
| Discovery Information | |
| Isolation Host | Microbacterium sp. Casco Bay |
| Found By | Sam Cousins |
| Year Found | 2024 |
| Location Found | South Portland, ME United States |
| Finding Institution | Southern Maine Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 43.645167 N, 70.227596 W Map |
| Discovery Notes | As the name suggests, Wrackline was isolated from a sample of sand taken above the wrack line of Willard beach - where sand, kelp, and other debris just touch with the grass. |
| Naming Notes | The wrack line is the trail of debris that is formed at high tide and left behind as it recedes. This phage was found just above the wrack line and, as SMCC enjoys working with marine phage, it seemed fitting to name it about some ocean phenomena. I'd also like to give our lab manager her due credit for she suggested the name - I simply affirmed it. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 5885 |
| Genome length (bp) | 42148 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | GF |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | Southern Maine Community College |
| Annotation Status | QC Complete |
| Plaque Notes | Wrackline forms plaques 0.5mm diameter on average although they have been observed to fluctuate in size. Interestingly, the plaques it forms are often surrounded by a halo - radiating about 0.3mm outward from the edge of the plaque. There is often a very faint ring of bacteria in the center of the plaque that looks very much like a bull's eye. |
| Morphotype | Siphoviridae |
| Number of Genes | 80 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewWrackline_1 Wrackline_2 Wrackline_3 Wrackline_4 Wrackline_5 Wrackline_6 Wrackline_7 Wrackline_8 Wrackline_9 Wrackline_10 Wrackline_11 Wrackline_12 Wrackline_13 Wrackline_14 Wrackline_15 Wrackline_16 Wrackline_17 Wrackline_18 Wrackline_19 Wrackline_20 Wrackline_21 Wrackline_22 Wrackline_23 Wrackline_24 Wrackline_25 Wrackline_26 Wrackline_27 Wrackline_28 Wrackline_29 Wrackline_30 Wrackline_31 Wrackline_32 Wrackline_33 Wrackline_34 Wrackline_35 Wrackline_36 Wrackline_37 Wrackline_38 Wrackline_39 Wrackline_40 Wrackline_41 Wrackline_42 Wrackline_43 Wrackline_44 Wrackline_45 Wrackline_46 Wrackline_47 Wrackline_48 Wrackline_49 Wrackline_50 Wrackline_51 Wrackline_52 Wrackline_53 Wrackline_54 Wrackline_55 Wrackline_56 Wrackline_57 Wrackline_58 Wrackline_59 Wrackline_60 Wrackline_61 Wrackline_62 Wrackline_63 Wrackline_64 Wrackline_65 Wrackline_66 Wrackline_67 Wrackline_68 Wrackline_69 Wrackline_70 Wrackline_71 Wrackline_72 Wrackline_73 Wrackline_74 Wrackline_75 Wrackline_76 Wrackline_77 Wrackline_78 Wrackline_79 Wrackline_80 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| GenBank File for Phamerator | Download |