Gordonia phage HannahD
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Detailed Information for Phage HannahD
Discovery Information
Isolation HostGordonia terrae 3612
Found ByHannah Dunnous
Year Found2018
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature30°C
GPS Coordinates40.2633 N, 79.5725 W Map
Discovery NotesThe bacteriophage was obtained from a flower bed outside Litchfield Tower C on The University of Pittsburgh campus in Pittsburgh. The soil collected in the sample was fine and dry, and some rocks and dried up roots were also present in the sample. It was 81 degrees Fahrenheit outside, and the humidity was 69%. My soil sample was then put into a conical tube with phage buffer and was put on the shaker for 2 days. The soil and phage buffer mixture was then filtered to isolate phage particles. The isolated phage particles were then plated on an agar plate with bacterial medium host Godonia terrae 3612.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 15, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage614
Genome length (bp)46095
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGCCGCGGTA
GC Content66.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDB
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes70
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK878901
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 90
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload