| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Esther Jakubowicz, Keverene Mason |
| Year Found | 2017 |
| Location Found | Suffern, NY USA |
| Finding Institution | Rockland Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.13363 N, 74.086268 W Map |
| Discovery Notes | Sept 18, 2017, 12 pm. Sunny day, temps at 24 degrees Celsius. Collected from Peace Garden, on RCC campus at approx. 6 cm deep. |
| Naming Notes | We named it after our Project's group name, Helpful Pests. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 5, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 947 |
| Genome length (bp) | 64794 |
| Character of genome ends | Circularly Permuted |
| GC Content | 59.6% |
| Sequencing Notes | sample contains a mixed population, 2% of reads are Cluster B. Sample will need to be purified and re-archived before annotation
at position 6809 bp approximately 20% of reads contain a T instead of consensus C. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | D |
| Subcluster | D1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Two morphologies noted. One larger, oval shaped 2 mm. The second one is a smaller, round, pinpoint plaque 1 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN284908 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
66 |
| Pitt Freezer Box Grid# |
H11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
