| Detailed Information for Phage Helpful | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Esther Jakubowicz, Keverene Mason |
| Year Found | 2017 |
| Location Found | Suffern, NY USA |
| Finding Institution | Rockland Community College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 41.13363 N, 74.086268 W Map |
| Discovery Notes | Sept 18, 2017, 12 pm. Sunny day, temps at 24 degrees Celsius. Collected from Peace Garden, on RCC campus at approx. 6 cm deep. |
| Naming Notes | We named it after our Project's group name, Helpful Pests. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 5, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 947 |
| Genome length (bp) | 64794 |
| Character of genome ends | Circularly Permuted |
| GC Content | 59.6% |
| Sequencing Notes | sample contains a mixed population, 2% of reads are Cluster B. Sample will need to be purified and re-archived before annotation at position 6809 bp approximately 20% of reads contain a T instead of consensus C. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | D |
| Subcluster | D1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | Two morphologies noted. One larger, oval shaped 2 mm. The second one is a smaller, round, pinpoint plaque 1 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 91 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewHelpful_1 Helpful_2 Helpful_3 Helpful_4 Helpful_5 Helpful_6 Helpful_7 Helpful_8 Helpful_9 Helpful_10 Helpful_11 Helpful_12 Helpful_13 Helpful_14 Helpful_15 Helpful_16 Helpful_17 Helpful_18 Helpful_19 Helpful_20 Helpful_21 Helpful_22 Helpful_23 Helpful_24 Helpful_25 Helpful_26 Helpful_27 Helpful_28 Helpful_29 Helpful_30 Helpful_31 Helpful_32 Helpful_33 Helpful_34 Helpful_35 Helpful_36 Helpful_37 Helpful_38 Helpful_39 Helpful_40 Helpful_41 Helpful_42 Helpful_43 Helpful_44 Helpful_45 Helpful_46 Helpful_47 Helpful_48 Helpful_49 Helpful_50 Helpful_51 Helpful_52 Helpful_53 Helpful_54 Helpful_55 Helpful_56 Helpful_57 Helpful_58 Helpful_59 Helpful_60 Helpful_61 Helpful_62 Helpful_63 Helpful_64 Helpful_65 Helpful_66 Helpful_67 Helpful_68 Helpful_69 Helpful_70 Helpful_71 Helpful_72 Helpful_73 Helpful_74 Helpful_75 Helpful_76 Helpful_77 Helpful_78 Helpful_79 Helpful_80 Helpful_81 Helpful_82 Helpful_83 Helpful_84 Helpful_85 Helpful_86 Helpful_87 Helpful_88 Helpful_89 Helpful_90 Helpful_91 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN284908 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 66 |
| Pitt Freezer Box Grid# | H11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
