| Detailed Information for Phage Hiddenleaf |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Daekwon Sequira |
| Year Found | 2018 |
| Location Found | Garnet Valley, PA USA |
| Finding Institution | Neumann University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.853972 N, 75.479417 W Map |
| Discovery Notes | Found in herb garden at the base of a bird bath. Responds well to the sound of R&B and Classical music |
| Naming Notes | The name came from when the agar was chunky the first time it was plated. But Since chunky was taken I change it to HiddenLeaf. Because the soil was found under a bird bath hidden under the leaves of a chive plant. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 28, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1334 |
| Genome length (bp) | 56082 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Neumann University |
| Annotation Status | In GenBank |
| Plaque Notes | Plaque morphology: Small exact size in diameter 2mm, The plaques were small and clear.
Phage morphology: Head: 53.63 nm, Tail length 100 nm. |
| Morphotype | Siphoviridae |
| Number of Genes | 84 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN497954 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.0*10^9 |
| Pitt Freezer Box# |
86 |
| Pitt Freezer Box Grid# |
H6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
