Microbacterium phage Chivey
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Detailed Information for Phage Chivey
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByPatricia Fallest-Strobl
Year Found2018
Location FoundGarnet Valley, PA USA
Finding InstitutionNeumann University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates39.853972 N, 75.479417 W Map
Discovery NotesBackyard herb garden at base of bird bath surrounded by chives.
Naming NotesSoil sample collected 2-3 cm deep among the roots of chive plants. In the lab, two small pill bugs were noted crawling around in the soil sample inside the ziploc bag.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 21, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2383
Genome length (bp)56082
Character of genome endsCircularly Permuted
GC Content63.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEF
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNeumann University
Annotation StatusBeing Annotated (Expected completion by 2/21/2020)
Plaque NotesThe direct plate showed 45 plaques of varying morphology. Plaque showed a clear middle with cloudy edges approximately 1-1.5 cm in diameter after incubation for 48 hours at 30C.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.3*10^9
Pitt Freezer Box# 86
Pitt Freezer Box Grid# H3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload