| Detailed Information for Phage Hiro | |
| Discovery Information | |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Brittany Sisson |
| Year Found | 2014 |
| Location Found | Boston, MA USA |
| Finding Institution | Florida Gulf Coast University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.347053 N, 71.134128 W Map |
| Discovery Notes | Hiro was isolated from a sample collected in the Boston area (90 Brainerd Road, Boston, MA 02134, USA). The sample was gathered and shipped to me by FGCU alumni, Michaela Smith. |
| Naming Notes | Hiro got its name from my favorite character on the television series, Heroes. The meaning of the name literally translates to "prosperous." I named my phage accordingly because although I failed nearly 30 times before finding Hiro, I ended up with one of the most sturdy lytic phage found within the class. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 5, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2220 |
| Genome length (bp) | 46854 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGTGAT |
| End Determination Notes | Read buildups in Illumina sequencing data. |
| GC Content | 58.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAlatin Alpacados AngryOrchard AppleCloud Belenaria BobbyDazzler Bonanza Bradshaw Bryce CosmicSans Dinger Erik Espica Gollum Harlequin Hiro Jester Krishelle Lillie Naiad Nancinator Natosaleda Partridge PhailMary Phrankenstein Rasputin RER2 RexFury RGL3 Rhodalysa Shuman StCroix Swann Takoda TWAMP UhSalsa Yogi Yoncess |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Currently, Hiro has a titer of 1.0 x 10^8 pfu/mL and a diameter of 2.5mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 65 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewHiro_1 Hiro_2 Hiro_3 Hiro_4 — tRNA Hiro_5 — tRNA Hiro_6 — tRNA Hiro_7 Hiro_8 Hiro_9 Hiro_10 Hiro_11 Hiro_12 Hiro_13 Hiro_14 Hiro_15 Hiro_16 Hiro_17 Hiro_18 Hiro_19 Hiro_20 Hiro_21 Hiro_22 Hiro_23 Hiro_24 Hiro_25 Hiro_26 Hiro_27 Hiro_28 Hiro_29 Hiro_30 Hiro_31 Hiro_32 Hiro_33 Hiro_34 Hiro_35 Hiro_36 Hiro_37 Hiro_38 Hiro_39 Hiro_40 Hiro_41 Hiro_42 Hiro_43 Hiro_44 Hiro_45 Hiro_46 Hiro_47 Hiro_48 Hiro_49 Hiro_50 Hiro_51 Hiro_52 Hiro_53 Hiro_54 Hiro_55 Hiro_56 Hiro_57 Hiro_58 Hiro_59 Hiro_60 Hiro_61 Hiro_62 Hiro_63 Hiro_64 Hiro_65 Hiro_66 Hiro_67 Hiro_68 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF324898 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.0 x 10^8 pfu/mL |
| Date of SEA Lysate Titering | Oct 31, 2014 |
| Pitt Freezer Box# | 124 |
| Pitt Freezer Box Grid# | C9 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |