| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Bella Canby, Jessica Castillo |
| Year Found | 2023 |
| Location Found | Emmitsburg, MD United States |
| Finding Institution | Mount St. Mary’s University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 39.6826 N, 77.34617 W Map |
| Discovery Notes | In collection for the bacteriophage JellyBread, it was collected by the powerplant sidewalk. The sample collected was a dry sample, with a clay-like consistency, and scooped 1-2 inches from the surface. |
| Naming Notes | Since the beginning of the phage lab, we have named every sample, agar plate, and lysate tube with the initials JB, for Jessica and Bella. Since we have discovered this phage together, it would only make sense if we shared equal custody of it. JellyBread contains
both J and B and who doesn’t like
Jelly and Bread? |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 3390 |
| Genome length (bp) | 53425 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 9 bases |
| Overhang Sequence | CGCCGGTGA |
| GC Content | 62.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Mount St. Mary’s University |
| Annotation Status | QC Complete |
| Plaque Notes | Bacteriophage JellyBread displays plaques with round, small, and cloudy plaques. The diameter of each plaque ranges from 1-2 millimeters. |
| Morphotype | Siphoviridae |
| Number of Genes | 93 |
| Number of tRNAs | 2 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| GenBank File for Phamerator | Download |