| Discovery Information |
| Isolation Host | Microbacterium paraoxydans NRRL B-14843 |
| Found By | Jacob Kinneman, Joe Alexander |
| Year Found | 2018 |
| Location Found | Hudson, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 28°C |
| GPS Coordinates | 44.98444 N, 92.7544 W Map |
| Discovery Notes | Dr. Klyczek Compost soil sample.
Compost pile next to garage in alley. Partially degraded leaves and kitchen scraps.
Soil was moist with partially decomposed leaves, grass, and vegetables.
Weather was sunny, clear, and no breeze. |
| Naming Notes | Random thought |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2051 |
| Genome length (bp) | 62439 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 1807 bp |
| End Determination Notes | Reads and draft sequence were run through PAUSE. There was a clear double-coverage region, and the right side of it had a well-defined end with a large buildup of reads. The left end, however, did not a have a clear single base where it ended, so the left end was chosen based on the approximate spot where the coverage dropped off. |
| GC Content | 58.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | GB |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Small, clear, no halo, and uniform |
| Morphotype | Siphoviridae |
| Number of Genes | 131 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN096358 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
87 |
| Pitt Freezer Box Grid# |
H3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
