| Detailed Information for Phage Limpid |
| Discovery Information |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Kimberly Anderson |
| Year Found | 2018 |
| Location Found | Denton, TX USA |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.211282 N, 97.148059 W Map |
| Discovery Notes | The temperature was 22 degrees Celsius outside. The soil was moist and was a dark color. |
| Naming Notes | The name Limpid came from the HTL being clear and the word limpid is a synonym for clear. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 29, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 241 |
| Genome length (bp) | 131038 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 788 bp |
| GC Content | 47.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogeny testing was not needed since there were no formation of mesas in plaques. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | My plaques were round in shape and clear. They had an average diameter of 0.2mm. |
| Number of Genes | 236 |
| Number of tRNAs | 34 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN369754 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.8x10^9 pfu/ml |
| Pitt Freezer Box# |
75 |
| Pitt Freezer Box Grid# |
D8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
