Streptomyces phage Limpid
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Detailed Information for Phage Limpid
Discovery Information
Isolation HostStreptomyces sanglieri UNT16F27A
Found ByKimberly Anderson
Year Found2018
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates33.211282 N, 97.148059 W Map
Discovery NotesThe temperature was 22 degrees Celsius outside. The soil was moist and was a dark color.
Naming NotesThe name Limpid came from the HTL being clear and the word limpid is a synonym for clear.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 29, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage241
Genome length (bp)131038
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length788 bp
GC Content47.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBK
SubclusterBK1
Cluster Life CycleLytic
Lysogeny NotesLysogeny testing was not needed since there were no formation of mesas in plaques.
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesMy plaques were round in shape and clear. They had an average diameter of 0.2mm.
Number of Genes236
Number of tRNAs34
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Limpid_1

Limpid_2

Limpid_3

Limpid_4

Limpid_5

Limpid_6
(DNA methyltransferase)

Limpid_7

Limpid_8
(ThyX-like thymidylate synthase)

Limpid_9

Limpid_10

Limpid_11

Limpid_12

Limpid_13

Limpid_14 — tRNA
(codon recognized: ACC)

Limpid_15 — tRNA
(codon recognized: ACG)

Limpid_16 — tRNA
(codon recognized: ACA)

Limpid_17
(hydrolase)

Limpid_18
(glycosyltransferase)

Limpid_19
(glycosyltransferase)

Limpid_20
(ribonucleotide reductase)

Limpid_21
(LysM-like endolysin)

Limpid_22

Limpid_23

Limpid_24
(portal protein)

Limpid_25

Limpid_26
(capsid maturation protease)

Limpid_27
(major capsid protein)

Limpid_28

Limpid_29
(head-to-tail adaptor)

Limpid_30

Limpid_31

Limpid_32

Limpid_33
(major tail protein)

Limpid_34
(tail assembly chaperone)

Limpid_35

Limpid_36
(tape measure protein)

Limpid_37
(minor tail protein)

Limpid_38
(minor tail protein)

Limpid_39

Limpid_40
(minor tail protein)

Limpid_41
(minor tail protein)

Limpid_42

Limpid_43

Limpid_44

Limpid_45
(HNH endonuclease)

Limpid_46
(metallophosphoesterase)

Limpid_47

Limpid_48

Limpid_49

Limpid_50

Limpid_51

Limpid_52

Limpid_53
(DNA helicase)

Limpid_54

Limpid_55
(DNA primase)

Limpid_56
(ssDNA binding protein)

Limpid_57
(DnaE-like DNA polymerase III alpha)

Limpid_58

Limpid_59
(deoxyuridine triphosphatase)

Limpid_60

Limpid_61
(RecA-like DNA recombinase)

Limpid_62

Limpid_63

Limpid_64
(Holliday junction resolvase)

Limpid_65

Limpid_66

Limpid_67

Limpid_68
(exonuclease/helicase)

Limpid_69

Limpid_70
(RuvC-like resolvase)

Limpid_71

Limpid_72
(DprA-like ssDNA binding protein)

Limpid_73

Limpid_74
(glycosyltransferase)

Limpid_75
(glycosyltransferase)

Limpid_76
(nucleotidyltransferase)

Limpid_77

Limpid_78
(exonuclease)

Limpid_79
(deoxynucleoside monophosphate kinase)

Limpid_80
(phosphatase)

Limpid_81

Limpid_82

Limpid_83

Limpid_84

Limpid_85
(terminase)

Limpid_86

Limpid_87 — tRNA
(codon recognized: CCA)

Limpid_88 — tRNA
(codon recognized: CCA)

Limpid_89
(HNH endonuclease)

Limpid_90 — tRNA
(codon recognized: CAG)

Limpid_91 — tRNA
(codon recognized: AUC)

Limpid_92 — tRNA
(codon recognized: UCA)

Limpid_93

Limpid_94 — tRNA
(codon recognized: GAC)

Limpid_95

Limpid_96

Limpid_97

Limpid_98

Limpid_99

Limpid_100
(Ro-like RNA binding protein)

Limpid_101

Limpid_102

Limpid_103

Limpid_104

Limpid_105 — tRNA
(codon recognized: CGU)

Limpid_106 — tRNA
(codon recognized: AGA)

Limpid_107

Limpid_108

Limpid_109
(DnaQ-like DNA polymerase III subunit)

Limpid_110

Limpid_111

Limpid_112 — tRNA
(codon recognized: GCA)

Limpid_113 — tRNA
(codon recognized: AAG)

Limpid_114

Limpid_115

Limpid_116 — tRNA
(codon recognized: AUG)

Limpid_117 — tRNA
(codon recognized: AUG)

Limpid_118

Limpid_119

Limpid_120 — tRNA
(codon recognized: UUC)

Limpid_121

Limpid_122

Limpid_123 — tRNA
(codon recognized: UAC)

Limpid_124 — tRNA
(codon recognized: CAC)

Limpid_125 — tRNA
(codon recognized: UGC)

Limpid_126 — tRNA
(codon recognized: AAC)

Limpid_127 — tRNA
(codon recognized: CGA)

Limpid_128 — tRNA
(codon recognized: AGG)

Limpid_129 — tmRNA

Limpid_130

Limpid_131

Limpid_132

Limpid_133

Limpid_134 — tRNA
(codon recognized: CUC)

Limpid_135

Limpid_136

Limpid_137

Limpid_138 — tRNA
(codon recognized: AGC)

Limpid_139
(RNA ligase)

Limpid_140

Limpid_141

Limpid_142
(polynucleotide kinase)

Limpid_143

Limpid_144

Limpid_145

Limpid_146

Limpid_147 — tRNA
(codon recognized: CUA)

Limpid_148

Limpid_149

Limpid_150

Limpid_151

Limpid_152 — tRNA
(codon recognized: GUA)

Limpid_153 — tRNA
(codon recognized: UUG)

Limpid_154

Limpid_155

Limpid_156

Limpid_157

Limpid_158

Limpid_159

Limpid_160
(rIIA-like protein)

Limpid_161
(rIIB-like protein)

Limpid_162

Limpid_163

Limpid_164
(phosphoesterase)

Limpid_165

Limpid_166 — tRNA
(codon recognized: AAA)

Limpid_167

Limpid_168
(WhiB family transcription factor)

Limpid_169

Limpid_170

Limpid_171

Limpid_172 — tRNA
(codon recognized: AUA)

Limpid_173

Limpid_174

Limpid_175

Limpid_176 — tRNA
(codon recognized: GAG)

Limpid_177

Limpid_178 — tRNA
(codon recognized: GAA)

Limpid_179 — tRNA
(codon recognized: GGC)

Limpid_180

Limpid_181

Limpid_182

Limpid_183 — tRNA
(codon recognized: GGA)

Limpid_184

Limpid_185 — tRNA
(codon recognized: CAA)

Limpid_186

Limpid_187

Limpid_188

Limpid_189

Limpid_190

Limpid_191

Limpid_192

Limpid_193
(thioredoxin)

Limpid_194

Limpid_195

Limpid_196

Limpid_197

Limpid_198

Limpid_199

Limpid_200
(RNA ligase)

Limpid_201

Limpid_202

Limpid_203

Limpid_204

Limpid_205

Limpid_206

Limpid_207

Limpid_208

Limpid_209

Limpid_210
(Lsr2-like DNA bridging protein)

Limpid_211

Limpid_212

Limpid_213

Limpid_214

Limpid_215

Limpid_216

Limpid_217

Limpid_218

Limpid_219

Limpid_220

Limpid_221

Limpid_222

Limpid_223

Limpid_224
(hydrolase)

Limpid_225

Limpid_226

Limpid_227

Limpid_228
(phosphoesterase)

Limpid_229

Limpid_230

Limpid_231

Limpid_232

Limpid_233

Limpid_234

Limpid_235

Limpid_236

Limpid_237

Limpid_238

Limpid_239

Limpid_240

Limpid_241

Limpid_242

Limpid_243

Limpid_244

Limpid_245

Limpid_246

Limpid_247

Limpid_248

Limpid_249

Limpid_250

Limpid_251

Limpid_252

Limpid_253

Limpid_254

Limpid_255

Limpid_256

Limpid_257

Limpid_258

Limpid_259

Limpid_260

Limpid_261

Limpid_262

Limpid_263

Limpid_264

Limpid_265

Limpid_266

Limpid_267

Limpid_268

Limpid_269

Limpid_270

Limpid_271

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN369754
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.8x10^9 pfu/ml
Pitt Freezer Box# 75
Pitt Freezer Box Grid# D8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload