Mycobacterium phage Megatron06
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Detailed Information for Phage Megatron06
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Duplicates of this PhageEmmaandSkylar
Found ByLogan Dykstra, Megan VanGarven
Year Found2023
Location FoundOrange City, IA United States
Finding InstitutionNorthwestern College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature29°C
GPS Coordinates42.998 N, 96.0582 W Map
Discovery NotesPhage was isolated from butterfly garden soil on campus.

Phage was discovered by Logan Dykstra on a direct isolation plate. Megan VanGarven adopted the phage and continued working with it.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 9, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage502
Genome length (bp)69095
Character of genome endsCircularly Permuted
GC Content57.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterH
SubclusterH1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNorthwestern College
Annotation StatusIn GenBank
Plaque NotesClear ~.5mm plaques after incubated at 37 degrees C for 48 hours.
Number of Genes94
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Megatron06_1

Megatron06_2

Megatron06_3
(capsid decoration protein)

Megatron06_4
(HNH endonuclease)

Megatron06_5
(terminase)

Megatron06_6
(portal protein)

Megatron06_7

Megatron06_8

Megatron06_9

Megatron06_10

Megatron06_11

Megatron06_12
(capsid maturation protease)

Megatron06_13
(major capsid protein)

Megatron06_14

Megatron06_15

Megatron06_16
(minor capsid protein)

Megatron06_17
(major capsid protein)

Megatron06_18

Megatron06_19

Megatron06_20

Megatron06_21

Megatron06_22
(tail terminator)

Megatron06_23

Megatron06_24

Megatron06_25
(major tail protein)

Megatron06_26
(tail assembly chaperone)

Megatron06_27
(tail assembly chaperone)

Megatron06_28
(tape measure protein)

Megatron06_29
(minor tail protein)

Megatron06_30
(minor tail protein)

Megatron06_31
(minor tail protein)

Megatron06_32
(minor tail protein D-ala-D-ala carboxypeptidase)

Megatron06_33
(minor tail protein)

Megatron06_34

Megatron06_35

Megatron06_36
(lysin A)

Megatron06_37

Megatron06_38

Megatron06_39

Megatron06_40
(lysin B)

Megatron06_41

Megatron06_42

Megatron06_43

Megatron06_44

Megatron06_45

Megatron06_46

Megatron06_47

Megatron06_48

Megatron06_49

Megatron06_50

Megatron06_51

Megatron06_52

Megatron06_53

Megatron06_54

Megatron06_55

Megatron06_56

Megatron06_57

Megatron06_58

Megatron06_59

Megatron06_60

Megatron06_61

Megatron06_62
(DNA helicase)

Megatron06_63

Megatron06_64

Megatron06_65

Megatron06_66

Megatron06_67

Megatron06_68
(RecB-like exonuclease/helicase)

Megatron06_69

Megatron06_70

Megatron06_71

Megatron06_72

Megatron06_73
(ASCE ATPase)

Megatron06_74

Megatron06_75

Megatron06_76

Megatron06_77

Megatron06_78
(DnaE-like DNA polymerase III alpha)

Megatron06_79

Megatron06_80
(RuvC-like resolvase)

Megatron06_81

Megatron06_82

Megatron06_83

Megatron06_84

Megatron06_85

Megatron06_86

Megatron06_87

Megatron06_88

Megatron06_89

Megatron06_90

Megatron06_91

Megatron06_92

Megatron06_93
(DNA primase/polymerase)

Megatron06_94

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978840
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 173
Pitt Freezer Box Grid# C4
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload