| Detailed Information for Phage MenE | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Alexa Almanza |
| Year Found | 2023 |
| Location Found | San Juan, TX United States |
| Finding Institution | South Texas College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 26.185206 N, 98.141531 W Map |
| Discovery Notes | The sample for DIANA was collected from a tree that was recently planted about 4 years ago. DIANA was originally one phage, but two morphologies formed, which formed HecaD and MenE. |
| Naming Notes | The original sample, DIANA, is named after my mother, who passed away in 2019. Diana is a Roman goddess that is often considered a moon goddess. She is also often considered a triple deity, being merged with Hecate, the Roman goddess of the new moon, ghosts, and magic, and Selene, the personification of the moon. My mother adored the crescent moon and would tell me that if I ever needed her and she wasn’t there, that I could talk to the moon. I wanted to dedicate these phages to her so that I know she is with me every step of the way. The “E” in MenE stands for Elizabeth, my mother’s middle name. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 9, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 861 |
| Genome length (bp) | 38495 |
| Character of genome ends | Circularly Permuted |
| GC Content | 68.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | GA |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members | |
| Annotating Institution | South Texas College |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques with a fuzzy margin formed. They were medium in size and varied a bit throughout a plate. |
| Number of Genes | 66 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMenE_1 MenE_2 MenE_3 MenE_4 MenE_5 MenE_6 MenE_7 MenE_8 MenE_9 MenE_10 MenE_11 MenE_12 MenE_13 MenE_14 MenE_15 MenE_16 MenE_17 MenE_18 MenE_19 MenE_20 MenE_21 MenE_22 MenE_23 MenE_24 MenE_25 MenE_26 MenE_27 MenE_28 MenE_29 MenE_30 MenE_31 MenE_32 MenE_33 MenE_34 MenE_35 MenE_36 MenE_37 MenE_38 MenE_39 MenE_40 MenE_41 MenE_42 MenE_43 MenE_44 MenE_45 MenE_46 MenE_47 MenE_48 MenE_49 MenE_50 — tRNA MenE_51 MenE_52 MenE_53 MenE_54 MenE_55 MenE_56 MenE_57 MenE_58 MenE_59 MenE_60 MenE_61 MenE_62 MenE_63 MenE_64 MenE_65 MenE_66 MenE_67 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ362677 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 188 |
| Pitt Freezer Box Grid# | A11 |
| Available Files | |
| GenBank File for Phamerator | Download |