| Detailed Information for Phage Pakusa | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Kashmala Mahmood |
| Year Found | 2022 |
| Location Found | Sterling, VA United States |
| Finding Institution | George Mason University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.009392 N, 77.378712 W Map |
| Discovery Notes | The sample was collected from the side of a relatively clear lake on the morning of August 29th. The location was a few feet off from a rough path, which divided the water body from a steep hill. The soil was moist due to contact with the lake water (similar to damp silt), and the sample was taken within 2 inches of the surface. |
| Naming Notes | The name of the phage is a reference to the discoverer's mother and birth nations. It is a concatenation of the former's first three letters and the acronym of the latter. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1214 |
| Genome length (bp) | 88438 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DQ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | George Mason University |
| Annotation Status | Overdue (Expected completion by 1/31/2025) |
| Plaque Notes | Small (about 1 mm), turbid plaques. No halos were seen and it was assumed that the plaques were temperate. |
| Morphotype | Myoviridae |
| Number of Genes | 121 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPakusa_1 Pakusa_2 Pakusa_3 Pakusa_4 Pakusa_5 Pakusa_6 Pakusa_7 Pakusa_8 Pakusa_9 Pakusa_10 Pakusa_11 Pakusa_12 Pakusa_13 Pakusa_14 Pakusa_15 Pakusa_16 Pakusa_17 Pakusa_18 Pakusa_19 Pakusa_20 Pakusa_21 Pakusa_22 Pakusa_23 Pakusa_24 Pakusa_25 Pakusa_26 Pakusa_27 Pakusa_28 Pakusa_29 Pakusa_30 Pakusa_31 Pakusa_32 Pakusa_33 Pakusa_34 Pakusa_35 Pakusa_36 Pakusa_37 Pakusa_38 Pakusa_39 Pakusa_40 Pakusa_41 Pakusa_42 Pakusa_43 Pakusa_44 Pakusa_45 Pakusa_46 Pakusa_47 Pakusa_48 Pakusa_49 Pakusa_50 Pakusa_51 Pakusa_52 Pakusa_53 Pakusa_54 Pakusa_55 Pakusa_56 Pakusa_57 Pakusa_58 Pakusa_59 Pakusa_60 Pakusa_61 Pakusa_62 Pakusa_63 Pakusa_64 Pakusa_65 Pakusa_66 Pakusa_67 Pakusa_68 Pakusa_69 Pakusa_70 Pakusa_71 Pakusa_72 Pakusa_73 Pakusa_74 Pakusa_75 Pakusa_76 Pakusa_77 Pakusa_78 Pakusa_79 Pakusa_80 Pakusa_81 Pakusa_82 Pakusa_83 Pakusa_84 Pakusa_85 Pakusa_86 Pakusa_87 Pakusa_88 Pakusa_89 Pakusa_90 Pakusa_91 Pakusa_92 Pakusa_93 Pakusa_94 Pakusa_95 Pakusa_96 Pakusa_97 Pakusa_98 Pakusa_99 Pakusa_100 Pakusa_101 Pakusa_102 Pakusa_103 Pakusa_104 Pakusa_105 Pakusa_106 Pakusa_107 Pakusa_108 Pakusa_109 Pakusa_110 Pakusa_111 Pakusa_112 Pakusa_113 Pakusa_114 Pakusa_115 Pakusa_116 Pakusa_117 Pakusa_118 Pakusa_119 Pakusa_120 — tRNA Pakusa_121 Pakusa_122 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 161 |
| Pitt Freezer Box Grid# | E4 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
