| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Nishi Patel and Julie Galo |
| Year Found | 2022 |
| Location Found | Baltimore, MD United States of America |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.25422 N, 76.71014 W Map |
| Discovery Notes | We obtained our bacteriophage from a soil sample taken from UMBC grounds near the commons building. The sample was collected on 9/15/2022 at 11:40 a.m. We performed direct isolation on 9/15/2022. We incubated the plates for 24 hours and performed our first round of phage purification through plaque assay on 9/19/2022.
Description: Dark brown and slightly wet. The sample was collected with soil and some mulch.
Temperature: Warm (80°F)
Collection depth: Surface level (about 2 inches). |
| Naming Notes | The name Patelgo comes from the combination of the last names Patel and Galo. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 23, 2023 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 677 |
| Genome length (bp) | 130302 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 712 bp |
| GC Content | 47.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The S. mirabilis plate was incubated for a week, and no mesa was formed. It can be assumed that Patelgo is a virulent phage. This cannot be confirmed till the phage DNA is sequenced. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques from our phage are clear, with a cloudy ring in the middle. The plaques are oval-shaped and are about 1-3 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 239 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ995431 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
151 |
| Pitt Freezer Box Grid# |
D6 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
