Microbacterium phage Phonegingi
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Detailed Information for Phage Phonegingi
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Former namesPhagingi
Found ByNatalie McLean
Year Found2022
Location FoundHudson, NC United States
Finding InstitutionMitchell Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates35.82831 N, 81.43249 W Map
Discovery NotesPhonegingi was discovered in red clay soil taken from the bed of a rabbit pen/chicken coop. The surrounding temperature was 80 degrees Fahrenheit. The phage was discovered 1cm. deep in semi-moist soil. The environment is rural and sees heavy foot traffic. Some debris were present in the soil.
Naming NotesThe name Phonegingi is taken from a character of the same name originating from Dialtown: A Phone Dating Simulator. Phonegingi, a cryptid, acts as the player character whose perspective shapes the game's plot and interactions. The intended name of my phage was Phagingi, a portmanteau between 'phage' and 'Phonegingi,' better aligning with the game's naming rules. However, this had to be changed to conform with phage naming rules.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 28, 2022
Approximate Shotgun Coverage2516
Genome length (bp)39364
Character of genome endsCircularly Permuted
GC Content67.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterGA
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionMitchell Community College
Annotation StatusIn GenBank
Plaque NotesPhonegingi is an aggressive phage, having plaques that average 3mm. in diameter. The plaques are clear and distinct, being varied throughout each plate.
Number of Genes67
Number of tRNAs1
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Phonegingi_1
(terminase small subunit)

Phonegingi_2

Phonegingi_3

Phonegingi_4

Phonegingi_5

Phonegingi_6
(terminase large subunit)

Phonegingi_7
(portal protein)

Phonegingi_8

Phonegingi_9

Phonegingi_10

Phonegingi_11
(scaffolding protein)

Phonegingi_12
(major capsid protein)

Phonegingi_13

Phonegingi_14
(head-to-tail adaptor)

Phonegingi_15
(head-to-tail stopper)

Phonegingi_16

Phonegingi_17
(tail terminator)

Phonegingi_18
(major tail protein)

Phonegingi_19
(tail assembly chaperone)

Phonegingi_20
(tail assembly chaperone)

Phonegingi_21
(tape measure protein)

Phonegingi_22
(minor tail protein)

Phonegingi_23
(minor tail protein)

Phonegingi_24

Phonegingi_25
(minor tail protein)

Phonegingi_26

Phonegingi_27

Phonegingi_28
(endolysin)

Phonegingi_29

Phonegingi_30

Phonegingi_31
(holin)

Phonegingi_32

Phonegingi_33

Phonegingi_34

Phonegingi_35

Phonegingi_36

Phonegingi_37
(RuvC-like resolvase)

Phonegingi_38
(RecE-like exonuclease)

Phonegingi_39
(RecT-like DNA pairing protein)

Phonegingi_40

Phonegingi_41
(HNH endonuclease)

Phonegingi_42
(RepA-like replication initiator)

Phonegingi_43
(helicase loader)

Phonegingi_44
(DnaB-like dsDNA helicase)

Phonegingi_45
(SSB protein)

Phonegingi_46
(ParB-like nuclease domain protein)

Phonegingi_47
(acetyltransferase)

Phonegingi_48 — tRNA
(tRNA-Gly(tcc))

Phonegingi_49

Phonegingi_50

Phonegingi_51

Phonegingi_52
(helix-turn-helix DNA binding domain protein)

Phonegingi_53

Phonegingi_54

Phonegingi_55

Phonegingi_56

Phonegingi_57

Phonegingi_58
(DNA binding domain protein)

Phonegingi_59

Phonegingi_60

Phonegingi_61

Phonegingi_62

Phonegingi_63

Phonegingi_64

Phonegingi_65

Phonegingi_66

Phonegingi_67

Phonegingi_68

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR475255
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 150
Pitt Freezer Box Grid# D5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload