| Detailed Information for Phage Phreeze | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Rina Watanabe |
| Year Found | 2017 |
| Location Found | Los Angeles, CA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.04604 N, 118.29136 W Map |
| Discovery Notes | Phage Phreeze was isolated from moist soil collected on 10/08/17 in Korea Town (Los Angeles) by Rina Watanabe. Air temperature at time of collection was 20°C. Phreeze was isolated by direct plating at 30°C. Characterization was performed by Keith Mitchell. Midi-Lysate (Archive) Titer: 8.25*10^12 PFU/mL Date of High-Titer Lysate Titration: 11/29/17 MIMG103AL-F17 Instructor: Dr. Amanda Freise Teaching Assistant: Andrew Lund Undergraduate Assistant: Keith Mitchell Laboratory Support: Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker |
| Naming Notes | Named after course instructor Dr. Amanda Freise. Both the name of the instructor and the name of the phage are pronounced "freeze." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 2, 2018 |
| Genome length (bp) | 67906 |
| Character of genome ends | Unknown |
| GC Content | 57.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | H |
| Subcluster | H1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | small turbid plaques, 0.5mm |
| Morphotype | Siphoviridae |
| Number of Genes | 92 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewPhreeze_1 Phreeze_2 Phreeze_3 Phreeze_4 Phreeze_5 Phreeze_6 Phreeze_7 Phreeze_8 Phreeze_9 Phreeze_10 Phreeze_11 Phreeze_12 Phreeze_13 Phreeze_14 Phreeze_15 Phreeze_16 Phreeze_17 Phreeze_18 Phreeze_19 Phreeze_20 Phreeze_21 Phreeze_22 Phreeze_23 Phreeze_24 Phreeze_25 Phreeze_26 Phreeze_27 Phreeze_28 Phreeze_29 Phreeze_30 Phreeze_31 Phreeze_32 Phreeze_33 Phreeze_34 Phreeze_35 Phreeze_36 Phreeze_37 Phreeze_38 Phreeze_39 Phreeze_40 Phreeze_41 Phreeze_42 Phreeze_43 Phreeze_44 Phreeze_45 Phreeze_46 Phreeze_47 Phreeze_48 Phreeze_49 Phreeze_50 Phreeze_51 Phreeze_52 Phreeze_53 Phreeze_54 Phreeze_55 Phreeze_56 Phreeze_57 Phreeze_58 Phreeze_59 Phreeze_60 Phreeze_61 Phreeze_62 Phreeze_63 Phreeze_64 Phreeze_65 Phreeze_66 Phreeze_67 Phreeze_68 Phreeze_69 Phreeze_70 Phreeze_71 Phreeze_72 Phreeze_73 Phreeze_74 Phreeze_75 Phreeze_76 Phreeze_77 Phreeze_78 Phreeze_79 Phreeze_80 Phreeze_81 Phreeze_82 Phreeze_83 Phreeze_84 Phreeze_85 Phreeze_86 Phreeze_87 Phreeze_88 Phreeze_89 Phreeze_90 Phreeze_91 Phreeze_92 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN010756 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 58 |
| Pitt Freezer Box Grid# | F6 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |