| Detailed Information for Phage ReginaGlobina |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Trishana Richards |
| Year Found | 2024 |
| Location Found | Atlanta, GA USA |
| Finding Institution | Spelman College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 33.4446 N, 84.2445 W Map |
| Discovery Notes | The phage was initially discovered on a plate exhibiting both large and small clear plaque morphologies. To isolate the desired larger plaques, four rounds of purification were required to separate them from the smaller ones. The resulting large, clear plaques were distinctly more prominent and robust, marking the successful isolation of the phage. |
| Naming Notes | This phage embodies both the regal persistence and challenge encountered during its isolation. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 328 |
| Genome length (bp) | 195163 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 17716 bp |
| GC Content | 54.0% |
| Sequencing Notes | This sample was sequenced as part of a DOGEMS (deliberately mixed) sample. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | FC |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Spelman College |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Clear, large and round |
| Morphotype | Siphoviridae |
| Number of Genes | 320 |
| Number of tRNAs | 30 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
