| Detailed Information for Phage Rhodalysa | |
| Discovery Information | |
| Isolation Host | Rhodococcus erythropolis RIA 643 |
| Found By | Juliet Downie |
| Year Found | 2014 |
| Location Found | Williamsburg, VA USA |
| Finding Institution | College of William & Mary |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.282925 N, 76.664688 W Map |
| Discovery Notes | Soil sample was isolated from compost soil. It underwent two rounds of purification before beginning web lysis process. Phage measurements: Capsid diameter = 60-70 nm Tail length = 150-200 nm |
| Naming Notes | The bacterial host was Rhodococcus globerulus; the phage lyses the bacteria, so it is a "lyser" or "lysa" of "Rhoda", hence "Rhoda""lyser", or "Rhodalysa". I also liked how this name can be pronounced "rhoda-LEE-sah", so it rhymes with Mona Lisa. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 28, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2782 |
| Genome length (bp) | 46596 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCCGTGAT |
| GC Content | 58.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CA |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAlatin Alpacados AngryOrchard AppleCloud Belenaria BobbyDazzler Bonanza Bradshaw Bryce CosmicSans Dinger Erik Espica Gollum Harlequin Hiro Jester Krishelle Lillie Naiad Nancinator Natosaleda Partridge PhailMary Phrankenstein Rasputin RER2 RexFury RGL3 Rhodalysa Shuman StCroix Swann Takoda TWAMP UhSalsa Yogi Yoncess |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques appeared small, clear, and circular. |
| Morphotype | Siphoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewRhodalysa_1 Rhodalysa_2 Rhodalysa_3 Rhodalysa_4 — tRNA Rhodalysa_5 — tRNA Rhodalysa_6 — tRNA Rhodalysa_7 Rhodalysa_8 Rhodalysa_9 Rhodalysa_10 Rhodalysa_11 Rhodalysa_12 Rhodalysa_13 Rhodalysa_14 Rhodalysa_15 Rhodalysa_16 Rhodalysa_17 Rhodalysa_18 Rhodalysa_19 Rhodalysa_20 Rhodalysa_21 Rhodalysa_22 Rhodalysa_23 Rhodalysa_24 Rhodalysa_25 Rhodalysa_26 Rhodalysa_27 Rhodalysa_28 Rhodalysa_29 Rhodalysa_30 Rhodalysa_31 Rhodalysa_32 Rhodalysa_33 Rhodalysa_34 Rhodalysa_35 Rhodalysa_36 Rhodalysa_37 Rhodalysa_38 Rhodalysa_39 Rhodalysa_40 Rhodalysa_41 Rhodalysa_42 Rhodalysa_43 Rhodalysa_44 Rhodalysa_45 Rhodalysa_46 Rhodalysa_47 Rhodalysa_48 Rhodalysa_49 Rhodalysa_50 Rhodalysa_51 Rhodalysa_52 Rhodalysa_53 Rhodalysa_54 Rhodalysa_55 Rhodalysa_56 Rhodalysa_57 Rhodalysa_58 Rhodalysa_59 Rhodalysa_60 Rhodalysa_61 Rhodalysa_62 Rhodalysa_63 Rhodalysa_64 Rhodalysa_65 Rhodalysa_66 Rhodalysa_67 Rhodalysa_68 Rhodalysa_69 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KT375356 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 6.10 x 10^8 PFU/mL |
| Date of SEA Lysate Titering | Nov 4, 2014 |
| Pitt Freezer Box# | 9 |
| Pitt Freezer Box Grid# | G12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |