| Detailed Information for Phage Rie18 |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Joseph Enwereji and Samantha Young |
| Year Found | 2019 |
| Location Found | Aba, Nigeria |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 21°C |
| GPS Coordinates | 5.121797 N, 7.343372 E Map |
| Discovery Notes | The soil sample was collected from the village of Umule Ovungwn, in Aba, Abia State, Nigeria. The depth of the soil the sample was taken from was 3 cm deep in dry soil about 3.048 meters from a building. |
| Naming Notes | We named it Rie18 because 'rie' directly translates to eat in Igbo. Igbo is the language spoken in the village of Umule Ovungwn. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 10, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2860 |
| Genome length (bp) | 56435 |
| Character of genome ends | Circularly Permuted |
| GC Content | 63.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | North Carolina A & T State University |
| Annotation Status | In GenBank |
| Plaque Notes | It appears to be lytic, because of the clear edges around the plaques. The plaques have a diameter of about 1 mm. |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT522003 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.1 x 10^18 |
| Pitt Freezer Box# |
98 |
| Pitt Freezer Box Grid# |
D11 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |