| Detailed Information for Phage SCentae |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Svitlana Martynuk |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.445404 N, 80.013111 W Map |
| Discovery Notes | Phage was isolated from soil on the Three Rivers Trail in Pittsburgh right in front of the Carnegie Science Center. The Three River Trails runs, as one would guess, right next to the river, so the soil was very moist and teeming with bacteria, and the S. Centae phage. |
| Naming Notes | This name is inspired by my nick name alluding to 50 Cent, called "Svitty Cent". This name is unique just like my phage, which has a unique morphology. I just added the "ae" to Cent to give it a nice sound, and restricted Svitty to just "S." for the sake of pronunciation. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 15, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 665 |
| Genome length (bp) | 151316 |
| Character of genome ends | Circularly Permuted |
| GC Content | 66.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | DO |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | The S. Centae phage has a relatively unique morphology. The plaques formed vary from pin point, 0.7mm, and 1mm in diameter. Never in between, and after several plaque purifications, this morphology was concluded to be the same phage. |
| Morphotype | Myoviridae |
| Number of Genes | 233 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK977696 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3^10pfu/ml |
| Date of SEA Lysate Titering |
Oct 19, 2016 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
