| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Former names | CherryTR33 |
| Found By | Sean Avryl C. Villaver, Marie Louise Abbey Badiola |
| Year Found | 2023 |
| Location Found | Rosedale, MD United States of America |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 13°C |
| GPS Coordinates | 39.342948 N, 76.479588 W Map |
| Discovery Notes | Date/Time of Collection: 09/20/23, 12:30 AM
Depth: Approximately 2 inches below the surface
Ambient humidity: 86%
Hydration: The soil was slightly damp, most likely because dew was forming on the grass (the actual temperature was close to the dew point).
Amount of organic matter: Minimal organic matter, clay-like soil texture.
General surroundings: Soil was taken from the ground beside a cherry tree. |
| Naming Notes | SeresaTree is so named because it was collected from the ground beside a cherry tree. We also found success with a different phage (not studied) near a cherry tree in a different location. Therefore, we decided to pay homage to the trees near which our isolations succeeded. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 1, 2024 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 246 |
| Genome length (bp) | 130894 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 772 bp |
| GC Content | 47.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogeny assays produced no lysogens, implying that SeresaTree is a virulent phage, following the lytic lifecycle. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 12/31/2025) |
| Plaque Notes | Plaques from this phage are clear, circular, and small with smooth edges. Approximately 1.4 mm in diameter after incubating for 24 hours on Streptomyces scabiei at 30°C. |
| Morphotype | Siphoviridae |
| Number of Genes | 247 |
| Number of tRNAs | 32 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
171 |
| Pitt Freezer Box Grid# |
E7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
