Microbacterium phage Sparcetus
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Detailed Information for Phage Sparcetus
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByNicholas Jirku
Year Found2021
Location FoundStorrs Mansfield, CT United States
Finding InstitutionUniversity of Connecticut
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature20°C
GPS Coordinates41.997222 N, 72.643611 W Map
Discovery NotesFound in a wildflower field near a nature reserve by fellow classmate Nancy Fuller.
Naming NotesThe name Sparcetus comes from two words.

Sparse - the phage was hard to find, as there were multiple samples taken and many tests done.

Cetus - a constellation called the whale and a sea monster in greek mythology. This was included in the name as the phage was named the day after a day of heavy rainfall.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1699
Genome length (bp)56871
Character of genome endsCircularly Permuted
GC Content62.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterEF
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Connecticut
Annotation StatusIn GenBank
Plaque NotesThe plaques are clear, with no sign of a bullseye, turbid edges, or halo. The plaque itself is siphoviridae.
MorphotypeSiphoviridae
Number of Genes86
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Sparcetus_1

Sparcetus_2

Sparcetus_3

Sparcetus_4

Sparcetus_5

Sparcetus_6

Sparcetus_7

Sparcetus_8

Sparcetus_9

Sparcetus_10

Sparcetus_11
(terminase)

Sparcetus_12

Sparcetus_13
(portal protein)

Sparcetus_14
(capsid maturation protease)

Sparcetus_15

Sparcetus_16

Sparcetus_17
(scaffolding protein)

Sparcetus_18

Sparcetus_19
(major capsid protein)

Sparcetus_20

Sparcetus_21

Sparcetus_22

Sparcetus_23

Sparcetus_24
(tail terminator)

Sparcetus_25

Sparcetus_26

Sparcetus_27
(major tail protein)

Sparcetus_28

Sparcetus_29

Sparcetus_30
(tape measure protein)

Sparcetus_31
(minor tail protein)

Sparcetus_32
(minor tail protein)

Sparcetus_33

Sparcetus_34
(minor tail protein)

Sparcetus_35
(minor tail protein)

Sparcetus_36

Sparcetus_37
(endolysin)

Sparcetus_38

Sparcetus_39

Sparcetus_40

Sparcetus_41

Sparcetus_42
(DNA helicase)

Sparcetus_43

Sparcetus_44

Sparcetus_45

Sparcetus_46
(RecB-like exonuclease/helicase)

Sparcetus_47

Sparcetus_48

Sparcetus_49
(RecA-like DNA recombinase)

Sparcetus_50

Sparcetus_51
(DNA primase)

Sparcetus_52

Sparcetus_53

Sparcetus_54

Sparcetus_55
(oxidoreductase)

Sparcetus_56
(phosphatase)

Sparcetus_57
(oxidoreductase)

Sparcetus_58

Sparcetus_59

Sparcetus_60

Sparcetus_61
(DnaE-like DNA polymerase III alpha)

Sparcetus_62

Sparcetus_63
(DnaE-like DNA polymerase III alpha)

Sparcetus_64

Sparcetus_65
(glycosyltransferase)

Sparcetus_66

Sparcetus_67

Sparcetus_68

Sparcetus_69

Sparcetus_70

Sparcetus_71
(ThyX-like thymidylate synthase)

Sparcetus_72

Sparcetus_73

Sparcetus_74

Sparcetus_75

Sparcetus_76

Sparcetus_77
(RuvC-like resolvase)

Sparcetus_78

Sparcetus_79

Sparcetus_80

Sparcetus_81

Sparcetus_82

Sparcetus_83

Sparcetus_84

Sparcetus_85

Sparcetus_86

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON755183
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.96x10^11 pfu/mL
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload