Gordonia phage Sting
Sting EM Picture
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Detailed Information for Phage Sting
Discovery Information
Isolation HostGordonia rubripertincta NRRL B-16540
Former namesUCEA4
Found ByUmar Chaudhry and Elijah Abdourakib
Year Found2021
Location FoundDurham, NC United States
Finding InstitutionDurham Technical Community College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature14°C
GPS Coordinates36.082552 N, 78.901486 W Map
Discovery NotesSoil sample with phage was found under decomposing leaves. Soil sample was under one inch of leaf cover and soil was collected from 0.5-1 inch into the ground.
Naming NotesThe name was thought of after my previous two names were rejected and at the time all I could think for a name was "Sting"
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedApr 11, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage71
Genome length (bp)57947
Character of genome endsCircularly Permuted
GC Content65.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCP
Subcluster--
Cluster Life CycleUnknown
Other Cluster Members
Annotating InstitutionConnecticut State Community College Northwestern
Annotation StatusIn GenBank
Plaque Notes~1 mm in diameter. Plaques are very small and concentrated. Plaques are turbid. Oval-shaped with a small elongated 'tail' coming out of the side of the main plaque. Plaques were also hard to see in some titers, in some titers plaques showed up just fine while in others there were no plaques present.
MorphotypeSiphoviridae
Number of Genes90
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Sting_1

Sting_2
(terminase)

Sting_3
(HNH endonuclease)

Sting_4

Sting_5

Sting_6

Sting_7

Sting_8
(DNA binding protein)

Sting_9

Sting_10

Sting_11

Sting_12

Sting_13

Sting_14
(RusA-like resolvase)

Sting_15

Sting_16
(DnaE-like DNA polymerase III (alpha))

Sting_17

Sting_18
(DNA binding protein)

Sting_19

Sting_20
(DNA binding protein)

Sting_21

Sting_22

Sting_23
(DNA binding protein)

Sting_24

Sting_25

Sting_26

Sting_27

Sting_28
(DNA methyltransferase)

Sting_29
(ParB-like nuclease domain)

Sting_30

Sting_31
(DNA binding protein)

Sting_32

Sting_33

Sting_34

Sting_35

Sting_36

Sting_37

Sting_38

Sting_39

Sting_40
(portal protein)

Sting_41
(capsid maturation protease)

Sting_42

Sting_43
(major capsid hexamer protein)

Sting_44
(major capsid pentamer protein)

Sting_45

Sting_46

Sting_47

Sting_48
(major tail protein)

Sting_49
(head-to-tail adaptor)

Sting_50

Sting_51

Sting_52
(tail assembly chaperone)

Sting_53
(tail assembly chaperone)

Sting_54

Sting_55
(tape measure protein)

Sting_56
(minor tail protein)

Sting_57
(minor tail protein)

Sting_58

Sting_59

Sting_60

Sting_61

Sting_62
(minor tail protein)

Sting_63

Sting_64
(endolysin)

Sting_65
(holin)

Sting_66

Sting_67

Sting_68

Sting_69

Sting_70

Sting_71
(DNA binding protein)

Sting_72

Sting_73

Sting_74
(DNA binding protein)

Sting_75

Sting_76

Sting_77

Sting_78

Sting_79

Sting_80

Sting_81
(Cas4 exonuclease)

Sting_82
(ASCE ATPase)

Sting_83

Sting_84

Sting_85
(SSB protein)

Sting_86
(HNH endonuclease)

Sting_87
(HNH endonuclease)

Sting_88

Sting_89

Sting_90
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPQ201089
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.04 x 10^9
Pitt Freezer Box# 142
Pitt Freezer Box Grid# C9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
GenBank File for PhameratorDownload