Gordonia phage Terapin
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Detailed Information for Phage Terapin
Discovery Information
Isolation HostGordonia terrae 3612
Found ByPhillip Koman
Year Found2015
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.443778 N, 79.953068 W Map
Discovery NotesAlthough this phage was discovered by Phillip, I adopted it to do further purification. The soil was collected outside of the Cathedral of Learning in a flower bed. The temperature was around 66-77 degrees Fahrenheit and the soil collected was described to be "dry and crumbly."
Naming NotesAs a request from Joe and Wale, current University of Maryland students, this phage was named Terrapin. UMD's mascot is better known as "Testudo," but I couldn't name my phage that due to trademarks on this name. However, my partner, Cece, spelled the name wrong so now this phage's name is spelled with one r.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage1039
Genome length (bp)66611
Character of genome endsCircularly Permuted
GC Content59.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDG
SubclusterDG1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plague produces varying plaque sizes that ranged in 1-1.5 mm in diameter.
MorphotypeSiphoviridae
Number of Genes97
Number of tRNAs0
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

Click to View

Terapin_1
(terminase large subunit)

Terapin_2
(portal protein)

Terapin_3

Terapin_4

Terapin_5

Terapin_6

Terapin_7

Terapin_8

Terapin_9

Terapin_10
(minor capsid protein)

Terapin_11
(major capsid protein)

Terapin_12

Terapin_13

Terapin_14

Terapin_15

Terapin_16
(head-to-tail connector protein)

Terapin_17

Terapin_18

Terapin_19
(major tail protein)

Terapin_20
(tail assembly chaperone)

Terapin_21
(tail assembly chaperone)

Terapin_22
(tape measure protein)

Terapin_23
(minor tail protein)

Terapin_24
(minor tail protein)

Terapin_25
(minor tail protein)

Terapin_26

Terapin_27
(minor tail protein)

Terapin_28
(minor tail protein)

Terapin_29
(minor tail protein)

Terapin_30
(lysin A)

Terapin_31

Terapin_32

Terapin_33

Terapin_34

Terapin_35

Terapin_36

Terapin_37

Terapin_38

Terapin_39

Terapin_40

Terapin_41

Terapin_42

Terapin_43

Terapin_44

Terapin_45

Terapin_46

Terapin_47

Terapin_48

Terapin_49

Terapin_50

Terapin_51
(helicase)

Terapin_52
(HNH endonuclease)

Terapin_53

Terapin_54

Terapin_55
(DEAD-like helicase)

Terapin_56

Terapin_57
(RecA-like recombinase)

Terapin_58

Terapin_59

Terapin_60
(oxidoreductase)

Terapin_61
(methyltransferase)

Terapin_62

Terapin_63

Terapin_64

Terapin_65
(DNA polymerase III alpha subunit)

Terapin_66

Terapin_67
(DNA polymerase III alpha subunit)

Terapin_68

Terapin_69

Terapin_70

Terapin_71

Terapin_72

Terapin_73
(ThyX-like thymidylate synthase)

Terapin_74

Terapin_75

Terapin_76

Terapin_77

Terapin_78

Terapin_79

Terapin_80

Terapin_81
(resolvase)

Terapin_82

Terapin_83
(ribbon-helix-helix DNA binding domain protein)

Terapin_84
(DNA primase/polymerase)

Terapin_85

Terapin_86

Terapin_87

Terapin_88

Terapin_89

Terapin_90

Terapin_91

Terapin_92

Terapin_93

Terapin_94

Terapin_95

Terapin_96
(lysin B)

Terapin_97

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX557285
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.58 x 10^9
Date of SEA Lysate Titering Nov 3, 2015
Pitt Freezer Box# 3
Pitt Freezer Box Grid# A5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload