| Detailed Information for Phage Thumb | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Thom3001 |
| Found By | Ana Garcia |
| Year Found | 2017 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.0732 N, 118.4505 W Map |
| Discovery Notes | Phage Thom3001 was isolated from soil was collected from the ground (depth of 1 in). Taken from moist garden area from the "Hill" at UCLA. In close proximity to buildings and walkways. Phage was isolated via Direct plating at 30C. Characterization ws performed by Team Legendary Lysis (Brian Aguirre, Ana Garcia, Juhee Lim, Malcolm Phung) and Team Drop it Lytics Hot (Zilla Wentworth, Paola Nol Bernardino, Joel Laskow, Carlos Rojas). Name of Individual who collected sample: Ana Garcia Midi-Lysate (Archive) Titer: 1 x 10^11 Date of Midi-Lysate Titer: 5-11-2017 MIMG103AL-S17 Instructor: Dr. Jordan Moberg Parker Teaching Assistant: Ximena Ibarra Undergraduate Assistant: Amara Pang Laboratory Support: Dr. Krisanavane Reddi |
| Naming Notes | This phage was named Thumb because it sounds kind of like the previously rejected name and the instructor thought it was funny. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 10, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Genome length (bp) | 68408 |
| Character of genome ends | Unknown |
| GC Content | 57.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | H |
| Subcluster | H1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | 1mm clear |
| Morphotype | Siphoviridae |
| Number of Genes | 94 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewThumb_1 Thumb_2 Thumb_3 Thumb_4 Thumb_5 Thumb_6 Thumb_7 Thumb_8 Thumb_9 Thumb_10 Thumb_11 Thumb_12 Thumb_13 Thumb_14 Thumb_15 Thumb_16 Thumb_17 Thumb_18 Thumb_19 Thumb_20 Thumb_21 Thumb_22 Thumb_23 Thumb_24 Thumb_25 Thumb_26 Thumb_27 Thumb_28 Thumb_29 Thumb_30 Thumb_31 Thumb_32 Thumb_33 Thumb_34 Thumb_35 Thumb_36 Thumb_37 Thumb_38 Thumb_39 Thumb_40 Thumb_41 Thumb_42 Thumb_43 Thumb_44 Thumb_45 Thumb_46 Thumb_47 Thumb_48 Thumb_49 Thumb_50 Thumb_51 Thumb_52 Thumb_53 Thumb_54 Thumb_55 Thumb_56 Thumb_57 Thumb_58 Thumb_59 Thumb_60 Thumb_61 Thumb_62 Thumb_63 Thumb_64 Thumb_65 Thumb_66 Thumb_67 Thumb_68 Thumb_69 Thumb_70 Thumb_71 Thumb_72 Thumb_73 Thumb_74 Thumb_75 Thumb_76 Thumb_77 Thumb_78 Thumb_79 Thumb_80 Thumb_81 Thumb_82 Thumb_83 Thumb_84 Thumb_85 Thumb_86 Thumb_87 Thumb_88 Thumb_89 Thumb_90 Thumb_91 Thumb_92 Thumb_93 Thumb_94 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG962376 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 45 |
| Pitt Freezer Box Grid# | D5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
