| Detailed Information for Phage WaldoWhy |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jessica Garcia |
| Year Found | 2016 |
| Location Found | Manhattan, KS USA |
| Finding Institution | Kansas State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 10°C |
| GPS Coordinates | 39.045 N, 96.856 W Map |
| Discovery Notes | The sample was taken from a flower pot, 2 inches deep. The soil was 50 degrees F. It was moist and dark. The location was in a shaded area in someone's front yard. Junction City, KS |
| Naming Notes | He was so hard to find. WaldoWhy and I have a love-hate relationship. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 735 |
| Genome length (bp) | 64529 |
| Character of genome ends | Circularly Permuted |
| GC Content | 59.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | D |
| Subcluster | D1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Kansas State University |
| Annotation Status | In GenBank |
| Plaque Notes | Lytic phage with an average plaque diameter of 1 mm after 24 hours. Rounded edges. Small pinpoint colonies. |
| Morphotype | Siphoviridae |
| Number of Genes | 89 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494102 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.3 x (10^7) pfu/ml |
| Date of SEA Lysate Titering |
Dec 6, 2016 |
| Pitt Freezer Box# |
35 |
| Pitt Freezer Box Grid# |
A5 |
| Available Files |
| EM Picture | Download |
| GenBank File for Phamerator | Download |