Rhodococcus phage Yogi
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Detailed Information for Phage Yogi
Discovery Information
Isolation HostRhodococcus erythropolis RIA 643
Found BySpencer Lambert & Laura Beth Aulds
Year Found2015
Location FoundRichland Parish, LA USA
Finding InstitutionUniversity of Louisiana at Monroe
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates32.388700 N, 91.792800 W Map
Discovery NotesYogi was found under a decaying log(part of the sample was some of the decaying bark) on a levee of a pond underneath shade of cypress trees. The soil was mostly dry, maybe a little moisture. Dark colored soil.
Naming NotesWe named our phage Yogi because while we were collecting the soil sample there was a black bear 75 yards from us watching us take the sample.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedNov 23, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage4855
Genome length (bp)46930
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCCGTGAT
GC Content58.8%
Sequencing NotesThere is a polymorphism in the reads for this genome. At position 33068, 46% of the reads call an "A" instead of the reported consensus base "G".
Fasta file available?Yes: Download fasta file
Characterization
ClusterCA
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesThe plaques were medium, clear circles. The diameter of the circles were uniform throughout the plate. The plaques formed within 18 hours of the infection. The titer was 10^10.
MorphotypeSiphoviridae
Number of Genes66
Number of tRNAs3
Number of tmRNAs0
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKX712236
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 22
Pitt Freezer Box Grid# H12
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload