Microbacterium phage Burritobowl
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Detailed Information for Phage Burritobowl
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found BySara Hughes and Mahnoor Siddiqui
Year Found2019
Location FoundLanghorne, PA United States of America
Finding InstitutionUniversity of the Sciences (now St. Joseph's Univ)
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.13534 N, 74.8908 W Map
Discovery NotesDiscovered Next to a barbecue grill in a suburban area. The soil was moist, and it was dug up about 10 cm below the ground. The temperature outside was about 25 degrees Celsius when the soil was collected.
Naming NotesWe chose this name for our Phage due to the fact that “Burrito” was already taken as name for another Phage in the database. We found that this name was very cute as catchy, and it was the first thing to come to our minds.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 20, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage40
Genome length (bp)41417
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceTCTCCCGGCA
GC Content66.5%
Sequencing NotesThis phage was sequenced as part of a DOGEMS sample.
Fasta file available?Yes: Download fasta file
Characterization
ClusterEB
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of the Sciences (now St. Joseph's Univ)
Annotation StatusIn GenBank
Plaque NotesOur plaques are about 0.635 cm in both heigh and width. They are both Turgid and Bulls-eye.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Burritobowl_1

Burritobowl_2
(terminase small subunit)

Burritobowl_3
(terminase large subunit)

Burritobowl_4
(portal and MuF-like fusion protein)

Burritobowl_5
(capsid maturation protease)

Burritobowl_6
(major capsid protein)

Burritobowl_7

Burritobowl_8
(head-to-tail adaptor)

Burritobowl_9
(head-to-tail stopper)

Burritobowl_10

Burritobowl_11
(tail terminator)

Burritobowl_12
(major tail protein)

Burritobowl_13
(tail assembly chaperone)

Burritobowl_14
(tail assembly chaperone)

Burritobowl_15

Burritobowl_16
(tape measure protein)

Burritobowl_17
(minor tail protein)

Burritobowl_18
(minor tail protein)

Burritobowl_19

Burritobowl_20
(minor tail protein)

Burritobowl_21
(minor tail protein)

Burritobowl_22
(lysin A)

Burritobowl_23

Burritobowl_24

Burritobowl_25

Burritobowl_26

Burritobowl_27

Burritobowl_28

Burritobowl_29
(Cas4 family exonuclease)

Burritobowl_30

Burritobowl_31
(MazG-like nucleotide pyrophosphohydrolase)

Burritobowl_32
(thymidylate kinase)

Burritobowl_33
(recombination directionality factor)

Burritobowl_34

Burritobowl_35

Burritobowl_36
(thioredoxin)

Burritobowl_37
(holliday junction resolvase)

Burritobowl_38

Burritobowl_39

Burritobowl_40

Burritobowl_41
(DNA primase)

Burritobowl_42
(DNA polymerase I)

Burritobowl_43

Burritobowl_44

Burritobowl_45
(RNA polymerase sigma factor)

Burritobowl_46

Burritobowl_47

Burritobowl_48

Burritobowl_49
(ParB-like nuclease domain protein)

Burritobowl_50

Burritobowl_51

Burritobowl_52
(HNH endonuclease)

Burritobowl_53

Burritobowl_54

Burritobowl_55

Burritobowl_56

Burritobowl_57

Burritobowl_58
(dihydrofolate reductase)

Burritobowl_59

Burritobowl_60

Burritobowl_61
(thymidylate synthase)

Burritobowl_62

Burritobowl_63

Burritobowl_64

Burritobowl_65

Burritobowl_66

Burritobowl_67

Burritobowl_68

Burritobowl_72
(HNH endonuclease)

Burritobowl_73

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT639649
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.4X10^6 PFU/mL
Pitt Freezer Box# 98
Pitt Freezer Box Grid# C9
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload