| Detailed Information for Phage Gack | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Gabrielle Stack |
| Year Found | 2023 |
| Location Found | Willington, CT United States |
| Finding Institution | University of Connecticut |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.83765 N, 72.262379 W Map |
| Discovery Notes | Found by dumpster near apartment complex - compact soil that is consistently dry and in the shade |
| Naming Notes | The name of this plaque originally came from a combination of my first and last name, however, the morphology of this plaque has an irregular edge, similar to a paint splatter, or goop, something that would make you exclaim GACK! |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1387 |
| Genome length (bp) | 40547 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | ACTCCCGGCA |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EB |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | We conducted a lysogeny test and it was found that this phage is lysogenic |
| Other Cluster Members |
Click to ViewAbigail Albedo Albright AnnaLie Armstrong Arroyo AvGardian Avocadoman BabyDaisy BabyYoda Bachaco BAjuniper BelmontSKP Bernstein Brahms BubbaBear Buldak Burritobowl Cashington Celaena ChiliPepper Clayda5 Coltrane CroZenni DickRichards Didgeridoo DirtyBubble Dismas Doobus Eden Elva Eula Finalfrontier FlameThrower Franklin22 Gack Icarian IndyLu Johnathan Jovita Katzastrophic Kenzers Kieran Lahqtemish LimaBean Loviatar Lynlen Nicky22 Phisb QMacho Quenya Rollins Rona SanaSana SansAfet SarBear Sharkboy Skylord Slay Stoor Stromboli Swervy TukTuk Vitas |
| Annotating Institution | University of Connecticut |
| Annotation Status | Being Annotated (Expected completion by 5/1/2024) |
| Plaque Notes | Plaque morphology is different at different dilutions, with larger cloudy clearings appearing around the irregular center clearings at higher dilutions. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewGack_Draft_1 Gack_Draft_2 Gack_Draft_3 Gack_Draft_4 Gack_Draft_5 Gack_Draft_6 Gack_Draft_7 Gack_Draft_8 Gack_Draft_9 Gack_Draft_10 Gack_Draft_11 Gack_Draft_12 Gack_Draft_13 Gack_Draft_14 Gack_Draft_15 Gack_Draft_16 Gack_Draft_17 Gack_Draft_18 Gack_Draft_19 Gack_Draft_20 Gack_Draft_21 Gack_Draft_22 Gack_Draft_23 Gack_Draft_24 Gack_Draft_25 Gack_Draft_26 Gack_Draft_27 Gack_Draft_28 Gack_Draft_29 Gack_Draft_30 Gack_Draft_31 Gack_Draft_32 Gack_Draft_33 Gack_Draft_34 Gack_Draft_35 Gack_Draft_36 Gack_Draft_37 Gack_Draft_38 Gack_Draft_39 Gack_Draft_40 Gack_Draft_41 Gack_Draft_42 Gack_Draft_43 Gack_Draft_44 Gack_Draft_45 Gack_Draft_46 Gack_Draft_47 Gack_Draft_48 Gack_Draft_49 Gack_Draft_50 Gack_Draft_51 Gack_Draft_52 Gack_Draft_53 Gack_Draft_54 Gack_Draft_55 Gack_Draft_56 Gack_Draft_57 Gack_Draft_58 Gack_Draft_59 Gack_Draft_60 Gack_Draft_61 Gack_Draft_62 Gack_Draft_63 Gack_Draft_64 Gack_Draft_65 Gack_Draft_66 Gack_Draft_67 Gack_Draft_72 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 183 |
| Pitt Freezer Box Grid# | A6 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |