Microbacterium phage Lyell
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Detailed Information for Phage Lyell
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Former namesWallus
Found ByKayla Doucette
Year Found2017
Location FoundRiver Falls, WI United States of America
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.85 N, 92.63 W Map
Discovery NotesCollected: 9-10-17
Location of collection: Glen Park, around 4 feet off the river bank of the Kinnickinnic River
Soil Condition: damp, very loose, a mixture of soil and sand granules
Soil Depth of collection: 130mm
Air temperature of location: 69 degrees Fahrenheit


Naming NotesThe first couple round of plaques showed that wallus had large, thicker plaque formations. So I thought of a Wallrus, and decided the name should be, Wallus.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage3168
Genome length (bp)62716
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3549 bp
GC Content61.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterED
SubclusterED2
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesSize - varies from 1mm-4mm
Appearance- clear circles (kind of odd shaped), not perfect circles and very light halos around clear plaque formations
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Lyell_1

Lyell_2

Lyell_3

Lyell_4

Lyell_5

Lyell_6

Lyell_7

Lyell_8

Lyell_9

Lyell_10
(membrane protein)

Lyell_11

Lyell_12

Lyell_13

Lyell_14
(DNA binding protein)

Lyell_15

Lyell_16

Lyell_17

Lyell_18

Lyell_19

Lyell_20

Lyell_21

Lyell_22

Lyell_23

Lyell_24

Lyell_25

Lyell_26

Lyell_30

Lyell_31

Lyell_32

Lyell_33

Lyell_34

Lyell_35
(NrdH-like glutaredoxin)

Lyell_36
(metallophosphoesterase)

Lyell_37

Lyell_38
(HNH endonuclease)

Lyell_39
(terminase)

Lyell_40
(portal protein)

Lyell_41
(capsid maturation protease)

Lyell_42

Lyell_43
(major capsid protein)

Lyell_44

Lyell_45

Lyell_46

Lyell_47
(hydrolase)

Lyell_48

Lyell_49

Lyell_50

Lyell_51

Lyell_52

Lyell_53

Lyell_54
(tail assembly chaperone)

Lyell_55
(tail assembly chaperone)

Lyell_56
(tape measure protein)

Lyell_57

Lyell_58
(minor tail protein)

Lyell_59

Lyell_60

Lyell_61

Lyell_62
(lysin A)

Lyell_63
(membrane protein)

Lyell_64
(membrane protein)

Lyell_65
(resolvase)

Lyell_66

Lyell_67

Lyell_68

Lyell_69

Lyell_70
(DNA primase)

Lyell_71

Lyell_72
(membrane protein)

Lyell_73

Lyell_74
(DNA helicase)

Lyell_75

Lyell_76

Lyell_77
(exonuclease)

Lyell_78

Lyell_79

Lyell_80
(RNA ligase)

Lyell_81
(polynucleotide kinase)

Lyell_82

Lyell_83

Lyell_84
(helicase)

Lyell_85

Lyell_86

Lyell_87

Lyell_88

Lyell_89

Lyell_90

Lyell_91
(membrane protein)

Lyell_92
(membrane protein)

Lyell_93

Lyell_94

Lyell_95
(membrane protein)

Lyell_96

Lyell_97

Lyell_98

Lyell_99

Lyell_100

Lyell_101

Lyell_102
(helix-turn-helix DNA binding domain protein)

Lyell_103

Lyell_104

Lyell_105

Lyell_106

Lyell_107

Lyell_108

Lyell_109
(membrane protein)

Lyell_110

Lyell_111

Lyell_112

Lyell_113

Lyell_114

Lyell_115

Lyell_116

Lyell_117

Lyell_118

Lyell_119

Lyell_120

Lyell_121

Lyell_122

Lyell_123

Lyell_124

Lyell_125
(membrane protein)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH371109
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 69
Pitt Freezer Box Grid# G7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload