| Detailed Information for Phage Lupine | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Former names | N/A |
| Found By | Natalie Hanson and Leah Watson |
| Year Found | 2018 |
| Location Found | Oxford, OH USA |
| Finding Institution | Miami University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.537222 N, 84.7325 W Map |
| Discovery Notes | The sample was taken from under pine straw around a conservation area sign for Helen S. Ruder Preserve across from Yager Stadium. |
| Naming Notes | Our original sample did not produce plaques, and our second sample disitegrated in a biodegradable flask during enrichment; therefore, our professor gave us a sample taken from July 2018. The sample was taken from pine straw. For this reason, our name of our phage is Lupine for the "pine" straw. Also, Leah's favorite Harry Potter chapter is Professor Lupus; this inspired our name, too. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1494 |
| Genome length (bp) | 62533 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3260 bp |
| GC Content | 62.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | ED |
| Subcluster | ED1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Our phage was most likely lytic, as the bacteria was completely lysed and the plaques were clear in the middle. |
| Other Cluster Members |
Click to ViewWolfstar (None) Alleb (ED1) DejaVu (ED1) Hortus1 (ED1) Hubbs (ED1) Jacko (ED1) Lupine (ED1) OlinDD (ED1) Pavlo (ED1) PhillyPhilly (ED1) Pioneer3 (ED1) Platte (ED1) Roman (ED1) Tandem (ED1) ASegato (ED2) DustyDino (ED2) Erenyeager (ED2) Fork (ED2) Lyell (ED2) Musetta (ED2) Necrophoxinus (ED2) RunningBrook (ED2) StevieWelch (ED2) Welcome (ED2) Yuma (ED2) |
| Annotating Institution | Miami University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque appeared to have a double ring. The small, outer ring is turbid, while the larger, inner ring is clear. The plaques all had similar morphology with defined borders. The plaque morphology and shape remained constant throughout the entire experiment. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLupine_1 Lupine_2 Lupine_3 Lupine_4 Lupine_5 Lupine_6 Lupine_7 Lupine_8 Lupine_9 Lupine_10 Lupine_11 Lupine_12 Lupine_13 Lupine_14 Lupine_15 Lupine_16 Lupine_17 Lupine_18 Lupine_19 Lupine_20 Lupine_21 Lupine_22 Lupine_23 Lupine_24 Lupine_25 Lupine_26 Lupine_27 Lupine_28 Lupine_29 Lupine_30 Lupine_31 Lupine_32 Lupine_33 Lupine_34 Lupine_35 Lupine_36 Lupine_37 Lupine_38 Lupine_39 Lupine_40 Lupine_41 Lupine_42 Lupine_43 Lupine_44 Lupine_45 Lupine_46 Lupine_47 Lupine_48 Lupine_49 Lupine_50 Lupine_51 Lupine_52 Lupine_53 Lupine_54 Lupine_55 Lupine_56 Lupine_57 Lupine_58 Lupine_59 Lupine_60 Lupine_61 Lupine_62 Lupine_63 Lupine_64 Lupine_65 Lupine_66 Lupine_67 Lupine_68 Lupine_69 Lupine_70 Lupine_71 Lupine_72 Lupine_73 Lupine_74 Lupine_75 Lupine_76 Lupine_77 Lupine_78 Lupine_79 Lupine_80 Lupine_81 Lupine_82 Lupine_83 Lupine_84 Lupine_85 Lupine_86 Lupine_87 Lupine_88 Lupine_89 Lupine_90 Lupine_91 Lupine_92 Lupine_93 Lupine_94 Lupine_95 Lupine_96 Lupine_97 Lupine_98 Lupine_99 Lupine_100 Lupine_101 Lupine_102 Lupine_103 Lupine_104 Lupine_105 Lupine_106 Lupine_107 Lupine_108 Lupine_109 Lupine_110 Lupine_111 Lupine_112 Lupine_113 Lupine_114 Lupine_115 Lupine_116 Lupine_117 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK919474 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.11x10^12 |
| Pitt Freezer Box# | 74 |
| Pitt Freezer Box Grid# | C10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
