| Detailed Information for Phage AnClar | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Kelsey White |
| Year Found | 2018 |
| Location Found | Ft. Myers, FL USA |
| Finding Institution | Florida Gulf Coast University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 26.460015 N, 81.780142 W Map |
| Discovery Notes | Discovered in the FGCU Food Forest. Found about 2 inches below mulch and dead vegetation, soil was somewhat moist. Soil was shaken in PYCa media (with no G. terrae) for a few hours and then shook for about 48 hours in G. terrae treated with 0.08 μm ciprofloxacin. The sample went through multiple rounds of ciprofloxacin selection. Ultimately, the ciprofloxacin concentration was raised to 0.2 μm and the phage was found to actively grow in the static G. terrae at this concentration. |
| Naming Notes | An Clár (Clare) is a county in Ireland where my family is from. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 14, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 751 |
| Genome length (bp) | 61856 |
| Character of genome ends | Circularly Permuted |
| GC Content | 69.8% |
| Sequencing Notes | In a subset of the population (about 28%), the 18 bases from 11871 to 11888 are duplicated, and the second copy appears immediately after the first. |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DR |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | Florida Gulf Coast University |
| Annotation Status | In GenBank |
| Plaque Notes | Small (about 1 mm), turbid plaques. |
| Number of Genes | 81 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAnClar_1 AnClar_2 AnClar_3 AnClar_4 AnClar_5 AnClar_6 AnClar_7 AnClar_8 AnClar_9 AnClar_10 AnClar_11 AnClar_12 AnClar_13 AnClar_14 AnClar_15 AnClar_16 AnClar_17 AnClar_18 AnClar_19 AnClar_20 AnClar_21 AnClar_22 AnClar_23 AnClar_24 AnClar_25 AnClar_26 AnClar_27 AnClar_28 AnClar_29 AnClar_30 AnClar_31 AnClar_32 AnClar_33 AnClar_34 AnClar_35 AnClar_36 AnClar_37 AnClar_38 AnClar_39 AnClar_40 AnClar_41 AnClar_42 AnClar_43 AnClar_44 AnClar_45 AnClar_46 AnClar_47 AnClar_48 AnClar_49 AnClar_50 AnClar_51 AnClar_52 AnClar_53 AnClar_54 AnClar_55 AnClar_56 AnClar_57 AnClar_58 AnClar_59 AnClar_60 AnClar_61 AnClar_62 AnClar_63 AnClar_64 AnClar_65 AnClar_66 AnClar_67 AnClar_68 AnClar_69 AnClar_70 AnClar_71 AnClar_72 AnClar_73 AnClar_74 AnClar_75 AnClar_76 AnClar_77 AnClar_78 AnClar_79 AnClar_80 AnClar_81 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN908693 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| GenBank File for Phamerator | Download |