| Detailed Information for Phage Angelique |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Angelique Ogunsemowo |
| Year Found | 2016 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 32°C |
| GPS Coordinates | 40.4439 N, 79.9575 W Map |
| Discovery Notes | -Weather conditions at time of soil collection: 72 degrees Farenheit, slight breeze
-Sample was transported for about 15 minutes prior to storage
-Soil conditions: moist, rich in mulch and plant debris |
| Naming Notes | This phage is the name of the founder. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 15, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1548 |
| Genome length (bp) | 50970 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CY |
| Subcluster | CY1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Hampden-Sydney College |
| Annotation Status | In GenBank |
| Plaque Notes | Phage Angelique was grown on the bacteria Gordonia terrae on a PYCa growth media, and incubated at 30°C for about 2 days.
Angelique produces round, “halo” looking plaques that can range in size from approximately 2-3mm.
|
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH651167 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3 x 10^11 pfu/ml |
| Date of SEA Lysate Titering |
Oct 10, 2016 |
| Pitt Freezer Box# |
42 |
| Pitt Freezer Box Grid# |
B3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
