| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Kip Cullinan and Kaarina Marttila |
| Year Found | 2021 |
| Location Found | Johnston, IA United States |
| Finding Institution | Northwestern College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 41.673144 N, 93.725921 W Map |
| Discovery Notes | This virus was discovered in a grassy region near a creek with a large amount of vegetation. The sample was obtained approximately 9 inches below the ground, and the area received a small amount of precipitation the previous evening. The soil was clumped, moist, and contained organic matter. |
| Naming Notes | This phage is named after my (Kip's) dog, Wrigley. As a life long Chicago Cubs fan, Wrigley was named after their stadium, Wrigley Field. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1445 |
| Genome length (bp) | 51878 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Northwestern College |
| Annotation Status | In GenBank |
| Plaque Notes | Wrigley produces medium sized, circular plaques that have distinct edges. In other words, the edges of Wrigley do not gradually fade into the bacteria; it has a definitive line of where it stops. Furthermore, Wrigley produces gray, hazy mesas that appear in the middle of the plaque, which is especially notable at high concentrations of the virus. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON108651 |
| Refseq Number | None yet |
| Published in Paper | Bastian et al 2022, Microbiology Resource Announcements: Complete Genome Sequences of Microbacterium Phages Clayda5 and Gshelby23 and Gordonia Phages Wrigley and Santhid |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
137 |
| Pitt Freezer Box Grid# |
B4 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
