Gordonia phage LonelyBoi
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Detailed Information for Phage LonelyBoi
Discovery Information
Isolation HostGordonia terrae 3612
Found ByJ. Axelson, E. Lider, S. Lord, & S. Wolf
Year Found2020
Location FoundSquirrel Hill, Pittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.426667 N, 79.905833 W Map
Discovery NotesThe soil sample was found in Frick Park near Dencreek and was given to us by a peer. Upon using this soil sample, we found it to be dry, had no evidence of fertilizer, was fine in texture, and did not have any large chunks of soil or wood.

N.B.: The full names of all those who discovered the phage are as follows:

Jessica Axelson, Ebru Lider, Samuel Lord, & Sarah Wolf
Naming NotesThis phage was named after the fact that it only appeared after extra days of incubation and only developed as one tiny, "lonely" clearing on the plate that we almost missed due to its size and "loneliness."
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 21, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1112
Genome length (bp)53604
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGTATGGCAT
GC Content66.4%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusBeing Annotated (Expected completion by 5/1/2021)
Plaque NotesProduces small(~ 1 mm in diameter), circular plaques that seem to be turbid around the edges
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 125
Pitt Freezer Box Grid# F12