Gordonia phage Cashline
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Detailed Information for Phage Cashline
Discovery Information
Isolation HostGordonia terrae 3612
Found ByColin Bashline
Year Found2019
Location FoundPittsburgh, PA United States of America
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature26°C
GPS Coordinates40.4448 N, 79.9606 W Map
Discovery NotesThe site of soil collection was the mulchy area right outside Irvis Hall, along the pathway leading to the entrance of the Peterson Sports Complex. Nearby vegetation is the planted trees. The topsoil appears dark and dry. The weather was temperate, 80 degrees Fahrenheit and sunny. The soil sample was taken approximately one inch below topsoil.
Naming NotesPhage was named from an anagram of the first letter of my (the discoverer's) first name replacing the first letter of my last name, a nickname I have had for years.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 10, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2164
Genome length (bp)52378
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGTATGGCAT
GC Content66.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCY
Subcluster--
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Pittsburgh
Annotation StatusIn GenBank
Plaque NotesPlaques are relatively small, around 2mm in average diameter. Plaques are entirely clear and relatively regular in roundness.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Cashline_1
(terminase, small subunit)

Cashline_2
(terminase, large subunit)

Cashline_3
(portal protein)

Cashline_4
(capsid maturation protease)

Cashline_5
(scaffolding protein)

Cashline_6
(capsid decoration protein)

Cashline_7
(major capsid protein)

Cashline_8

Cashline_9
(head-to-tail adaptor)

Cashline_10
(head-to-tail stopper)

Cashline_11

Cashline_12

Cashline_13
(tail terminator)

Cashline_14
(major tail protein)

Cashline_15
(tail assembly chaperone)

Cashline_16
(tail assembly chaperone)

Cashline_17

Cashline_18
(tape measure protein)

Cashline_19
(minor tail protein)

Cashline_20
(minor tail protein)

Cashline_21

Cashline_22
(lysin A, N-acetylmuramoyl-L-alanine amidase domain)

Cashline_23
(holin)

Cashline_24

Cashline_25

Cashline_26
(lysin B)

Cashline_27
(minor tail protein)

Cashline_28

Cashline_29
(minor tail protein)

Cashline_30

Cashline_31

Cashline_32
(minor tail protein)

Cashline_33

Cashline_34

Cashline_35

Cashline_36

Cashline_37
(Ro-like RNA binding protein)

Cashline_38
(tyrosine integrase)

Cashline_39
(helix-turn-helix DNA binding domain protein)

Cashline_40
(metallopeptidase)

Cashline_41
(immunity repressor)

Cashline_42
(helix-turn-helix DNA binding domain protein)

Cashline_43

Cashline_44

Cashline_45
(antirepressor)

Cashline_46

Cashline_47

Cashline_48
(excise)

Cashline_49

Cashline_50

Cashline_51

Cashline_52

Cashline_53

Cashline_54

Cashline_55

Cashline_56
(RecE-like exonuclease)

Cashline_57
(RecT-like ssDNA binding protein)

Cashline_58

Cashline_59

Cashline_60

Cashline_61

Cashline_62
(DNA methylase)

Cashline_63

Cashline_64
(helix-turn-helix DNA binding domain protein)

Cashline_65

Cashline_66

Cashline_67

Cashline_68

Cashline_69
(RepA-like replication initiator)

Cashline_70

Cashline_71

Cashline_72
(RusA-like resolvase)

Cashline_73

Cashline_74
(helix-turn-helix DNA binding domain protein)

Cashline_75

Cashline_76
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW534378
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 113
Pitt Freezer Box Grid# H8
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload