| Detailed Information for Phage Confidence |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Gwenyth Ballard, Kongmeng Lo, Christopher Rost |
| Year Found | 2016 |
| Location Found | River Falls, WI, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 29°C |
| GPS Coordinates | 44.852 N, 92.621 W Map |
| Discovery Notes | The soil was under a tent in wet conditions for over a week before it was collected as a sample. The Siphoviradae tail for the phage was quite long. Kong and Chris isolated the phage from Gwenyth's soil sample. |
| Naming Notes | Throughout the whole experience, we experience a lot of trial and error. Although, we had a lot of experimental error and re-trials, we never lost any confidence; thus, this was where the name was found. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jun 15, 2017 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 194 |
| Genome length (bp) | 50646 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CY |
| Subcluster | CY1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Small and Clear. No halos were present. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG872834 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.185x10^7 |
| Date of SEA Lysate Titering |
Oct 17, 2016 |
| Pitt Freezer Box# |
37 |
| Pitt Freezer Box Grid# |
E8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
