Detailed Information for Phage Confidence | |
Discovery Information | |
Isolation Host | Gordonia terrae 3612 |
Found By | Gwenyth Ballard, Kongmeng Lo, Christopher Rost |
Year Found | 2016 |
Location Found | River Falls, WI, WI USA |
Finding Institution | University of Wisconsin-River Falls |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | 29°C |
GPS Coordinates | 44.852 N, 92.621 W Map |
Discovery Notes | The soil was under a tent in wet conditions for over a week before it was collected as a sample. The Siphoviradae tail for the phage was quite long. Kong and Chris isolated the phage from Gwenyth's soil sample. |
Naming Notes | Throughout the whole experience, we experience a lot of trial and error. Although, we had a lot of experimental error and re-trials, we never lost any confidence; thus, this was where the name was found. |
Sequencing Information | |
Sequencing Complete? | Yes |
Date Sequencing Completed | Jun 15, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina |
Sequencer Used | Illumina MiSeq |
Read Type | Single-end reads |
Read Length | 150 bp |
Approximate Shotgun Coverage | 194 |
Genome length (bp) | 50646 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | CGTATGGCAT |
GC Content | 66.4% |
Fasta file available? | Yes: Download fasta file |
Characterization | |
Cluster | CY |
Subcluster | CY1 |
Cluster Life Cycle | Temperate |
Other Cluster Members | |
Annotating Institution | Unknown or unassigned |
Annotation Status | In GenBank |
Plaque Notes | Small and Clear. No halos were present. |
Morphotype | Siphoviridae |
Number of Genes | 79 |
Number of tRNAs | 0 |
Number of tmRNAs | 0 |
Has been Phamerated? | Yes |
Gene List |
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Submitted Minimal DNA Master File | Download |
Publication Info | |
Uploaded to GenBank? | Yes |
GenBank Accession | MG872834 |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Archived |
SEA Lysate Titer | 2.185x10^7 |
Date of SEA Lysate Titering | Oct 17, 2016 |
Pitt Freezer Box# | 37 |
Pitt Freezer Box Grid# | E8 |
Available Files | |
Plaque Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |