| Detailed Information for Phage DalanDe | |
| Discovery Information | |
| Isolation Host | Gordonia rubripertincta NRRL B-16540 |
| Found By | Erika Hall and Alyssa Finocchiaro |
| Year Found | 2020 |
| Location Found | Lakeland, FL US |
| Finding Institution | University of South Florida |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 27.955234 N, 82.007758 W Map |
| Discovery Notes | Collected on 09/10/2020, 75°F, 94% humidity, recent downpour, dew from morning, collected at 5:45am,and near a pond and creek that routinely floods. In area of frequent wildlife including but not limited to fish, birds, squirrels, otters, turtles, snakes, amphibians and reptiles, insects, trees, ferns, lily pads, vines, and other weeds. Area gets little sun, mostly shade due to surrounding trees, soil gathered from ground level to about an inch down, soil was very wet but not muddy, it clumped together well and was relatively easy to scoop up with the provided sticks but was not liquid or very loose. The soil was dark and cold. |
| Naming Notes | Our process of phage purification had a lot of twists and turns, our plaques turned from cloudy to clear, small to medium then back to small, and several experiments had to be redone due to contamination or messed up plates. We both thought of butterflies as a name inspiration due to their dramatic change and dalán-dé (also written as Daelan-dé) means butterfly in Scottish which also roughly translates to God's fire which is cool. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 22, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 560 |
| Genome length (bp) | 85762 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DQ |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | North Carolina A&T State University |
| Annotation Status | In GenBank |
| Plaque Notes | Small lytic plaques. These plaques are very small and difficult to see without holding the plate up to a light. Counting plaques is easiest by using a marker to count dots but the plaques do not translate well on camera. Occasionally a larger plaque will appear, especially in higher concentrations. This phage grows slower than average and take a high concentration to web. |
| Morphotype | Myoviridae |
| Number of Genes | 109 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDalanDe_1 DalanDe_2 DalanDe_3 DalanDe_4 DalanDe_5 DalanDe_6 DalanDe_7 DalanDe_8 DalanDe_9 DalanDe_10 DalanDe_11 DalanDe_12 DalanDe_13 DalanDe_14 DalanDe_15 DalanDe_16 DalanDe_17 DalanDe_18 DalanDe_19 DalanDe_20 DalanDe_21 DalanDe_22 DalanDe_23 DalanDe_24 DalanDe_25 DalanDe_26 DalanDe_27 DalanDe_28 DalanDe_29 DalanDe_30 DalanDe_31 DalanDe_32 DalanDe_33 DalanDe_34 DalanDe_35 DalanDe_36 DalanDe_37 DalanDe_38 DalanDe_39 DalanDe_40 DalanDe_41 DalanDe_42 DalanDe_43 DalanDe_44 DalanDe_45 DalanDe_46 DalanDe_47 DalanDe_48 DalanDe_49 DalanDe_50 DalanDe_51 DalanDe_52 DalanDe_53 DalanDe_54 DalanDe_55 DalanDe_56 DalanDe_57 DalanDe_58 DalanDe_59 DalanDe_60 DalanDe_61 DalanDe_62 DalanDe_63 DalanDe_64 DalanDe_65 DalanDe_66 DalanDe_67 DalanDe_68 DalanDe_69 DalanDe_70 DalanDe_71 DalanDe_72 DalanDe_73 DalanDe_74 DalanDe_75 DalanDe_76 DalanDe_77 DalanDe_78 DalanDe_79 DalanDe_80 DalanDe_81 DalanDe_82 DalanDe_83 DalanDe_84 DalanDe_85 DalanDe_86 DalanDe_87 DalanDe_88 DalanDe_89 DalanDe_90 DalanDe_91 DalanDe_92 DalanDe_93 DalanDe_94 DalanDe_95 DalanDe_96 DalanDe_97 DalanDe_98 DalanDe_99 DalanDe_100 DalanDe_101 DalanDe_102 DalanDe_103 DalanDe_104 DalanDe_105 DalanDe_106 DalanDe_107 DalanDe_108 DalanDe_109 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON755184 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 118 |
| Pitt Freezer Box Grid# | D12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |