| Detailed Information for Phage Destructrice | |
| Discovery Information | |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Paige & Kathryn |
| Year Found | 2024 |
| Location Found | Louisville, KY United States |
| Finding Institution | University of Evansville |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 81°C |
| GPS Coordinates | 38.20882 N, 85.75387 E Map |
| Discovery Notes | I obtained a soil sample from moist grass at GPS coordinates 38.20882 N, 85.75387 W in Louisville, Kentucky. I took the sample from about 4 inches below the surface. The sample was dark brown and moist, with an earthy smell. |
| Naming Notes | This is French for "destructor". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 4540 |
| Genome length (bp) | 45548 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 265 bp |
| GC Content | 59.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of Evansville |
| Annotation Status | In GenBank |
| Plaque Notes | We completed the enriched isolation by filtering the culture through a 0.22mm filter. We then spread the Streptomyces culture over the entire plate. For the negative control, 3uL of phage buffer was spotted onto one half of the plate. On the other half, 3uL of the filtered sample was spotted on the other division. After two day incubation, a region of clearing was observed on the section spread with the filtered sample (Figure 1B). The negative control or section spotted with phage buffer did not have a region of clearing indicating that the sample is accurate and has phage. These results suggest that the sample contained phage that can infect Streptomyces griseus. |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 20 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDestructrice_1 Destructrice_2 Destructrice_3 Destructrice_4 Destructrice_5 Destructrice_6 Destructrice_7 Destructrice_8 Destructrice_9 Destructrice_10 Destructrice_11 Destructrice_12 Destructrice_13 Destructrice_14 — tRNA Destructrice_15 — tRNA Destructrice_16 — tRNA Destructrice_17 — tRNA Destructrice_18 — tRNA Destructrice_20 — tRNA Destructrice_21 — tRNA Destructrice_22 — tRNA Destructrice_23 — tRNA Destructrice_24 — tRNA Destructrice_25 — tRNA Destructrice_26 — tRNA Destructrice_27 — tRNA Destructrice_28 — tRNA Destructrice_29 — tRNA Destructrice_30 — tRNA Destructrice_31 — tRNA Destructrice_32 — tRNA Destructrice_33 — tRNA Destructrice_34 — tRNA Destructrice_35 Destructrice_36 Destructrice_37 Destructrice_38 Destructrice_39 Destructrice_40 Destructrice_41 Destructrice_42 Destructrice_43 Destructrice_44 Destructrice_45 Destructrice_46 Destructrice_47 Destructrice_48 Destructrice_49 Destructrice_50 Destructrice_51 Destructrice_52 Destructrice_53 Destructrice_54 Destructrice_55 Destructrice_56 Destructrice_57 Destructrice_58 Destructrice_59 Destructrice_60 Destructrice_61 Destructrice_62 Destructrice_63 Destructrice_64 Destructrice_65 Destructrice_66 Destructrice_67 Destructrice_68 Destructrice_69 Destructrice_70 Destructrice_71 Destructrice_72 Destructrice_73 Destructrice_74 Destructrice_75 Destructrice_76 Destructrice_77 Destructrice_78 Destructrice_79 Destructrice_80 Destructrice_81 Destructrice_82 Destructrice_83 Destructrice_84 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PX234433 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 196 |
| Pitt Freezer Box Grid# | E12 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
