Streptomyces phage Percastrophe
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Detailed Information for Phage Percastrophe
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Former namesPercyverance
Found ByPercyval Seddoh
Year Found2017
Location FoundDenton, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates33.19747 N, 97.132884 W Map
Discovery NotesThe soil was clumpy when it was obtained from a grassy area near bushes away from walking areas. The soil was dark and slightly moist.
Naming NotesThis was my 5th soil sample additionally, I adopted the phage from a classmate. For me the most important part of science can at times to catastrophic, and I had to learn this lesson. I had 4 failures prior to this phage. I fused my name "Percyval" with "catastrophe" to make "Percastrophe".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 2, 2017
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)45999
Character of genome endsUnknown
Direct Terminal Repeat Length264 bp
End Determination NotesWhile one end of the terminal repeat was obvious in the sequencing reads for this project, the other end was far less so. A buildup of reads was observed at a position that matched the right side of the terminal repeat in other similar phages, so that was selected as the right end of the terminal repeat.
GC Content59.7%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesMy plaques are clear, circular, and approximately 3mm in diameter.
Number of Genes64
Number of tRNAs22
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG663583
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.0 x 10^9
Pitt Freezer Box# 70
Pitt Freezer Box Grid# D2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload