| Detailed Information for Phage Familton |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Daniel Shen |
| Year Found | 2016 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.075071 N, 118.450961 W Map |
| Discovery Notes | Direct Isolation at 30C. Sample collection and initial isolation by Daniel Shen. Purification and amplification by Samuel Wu.
Genome annotation performed by Jeffrey Zhao, Nathan Sumarsono, Max Borman, Andrew Pham, Christine Shieh, Nezia Rahman, Stephanie Gao, Katherine Lee. Annotation QC performed by Jordan Moberg Parker. DNA preparation performed by Krisanavane Reddi.
Instructor: Dr. Jordan Moberg Parker
Teaching Assistant: Michelle Bina
Undergraduate Assistant: Samuel Wu
EM shows long prolate head. |
| Naming Notes | Named after the hit musical, which is a favorite of one of the TAs. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 20, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 90 |
| Genome length (bp) | 71807 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTGT |
| GC Content | 65.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | 1mm pinpoint plaques |
| Morphotype | Siphoviridae |
| Number of Genes | 129 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MG099943 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
45 |
| Pitt Freezer Box Grid# |
C12 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
