Mycobacterium phage Zakhe101
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Detailed Information for Phage Zakhe101
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByZakhe M Mcunu
Year Found2014
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramMycobacterial Genetics Course, Durban, South Africa
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.817371 S, 30.942245 E Map
Discovery NotesThis phage was found near the laboratoy soil, the soil that it was extracted from was moist and dark. The colonies were so small and grow after a 24hr incubation at 37C. It is location 3A on the lysate prepared
Naming NotesThe name of this phage comes directly to my own name Zakhe meaning 'make'. This phage is morphologically different compare to other phages that were isolated as it had a prolate head and tail. the code 101 means that it usually have a spot that include plaques inside a rare feacture in the immunity test. Therefore its name is Zakhe101
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 29, 2015
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage215
Genome length (bp)69653
Character of genome ends3' Sticky Overhang
Overhang Length4 bases
Overhang SequenceGTGT
GC Content65.5%
Fasta file available?Yes: Download fasta file
Characterization
ClusterO
Subcluster--
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaques were a circular shape. the head of the phage is prolate similar to corndog and Dylan phages. From the digest picture it can be seen against Da Vinci on position 2 that it forms a clear spot.
MorphotypeSiphoviridae
Number of Genes123
Has been Phamerated?No
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT281796
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 7
Pitt Freezer Box Grid# C3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload