Mycobacterium phage Idergollasper
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Detailed Information for Phage Idergollasper
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByThientrinh Nguyen
Year Found2021
Location FoundWynnewood, PA United States of America
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates39.998871 N, 75.28797 W Map
Discovery NotesIdergollasper was found under a tree at a corner of South Ardmore Park between Sussex Road and Athens Avenue in Wynnewood, Pennsylvania. The soil sample was collected under a tree located closer to the side of Sussex Road by Julia Iacovella on Monday, January 25, 2021 at 3:00 pm. The depth of the soil was 13 cm, and the soil was hard and dry. The weather conditions when the soil sample was collected was cold and windy with overcast. The ambient temperature was 4.44 degrees Celsius and the ambient humidity was 50%.

The titer of the HTL is 1.7 x 10^9 pfu/mL. The concentration of Idergollasper's DNA is 203.0 ng/uL. The OD 260/280 ratio is 1.82.

The EM image shows that Idergollasper phage particles have a prolate capsid head and a non-contractile tail. Average Idergollasper phage particles measure 150 nm (capsid length), 25 nm (capsid width), and 275 nm (capsid tail), N=3.

Because Idergollasper's restriction digest is similar to other Cluster O's virtual digest, it is likely that Idergollasper belongs to Cluster O.
Naming NotesIdergollasper is the name of a random playlist that was suggested to me on Spotify. Idergollasper is also my study playlist, and it was playing when I was collecting one of my soil sample.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterO
Subcluster--
Cluster Life CycleLytic
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesIdergollasper forms small, clear plaques less than 1 mm in diameter with small, cloudy bumps surrounding each plaque. The plaques were formed after 24 to 72 hours in the incubator at 37 degrees Celsius.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload