Mycobacterium phage Idergollasper
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|Detailed Information for Phage Idergollasper|
|Isolation Host||Mycobacterium smegmatis mc²155|
|Found By||Thientrinh Nguyen|
|Location Found||Wynnewood, PA United States of America|
|Finding Institution||Saint Joseph's University|
|Program||Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science|
|From enriched soil sample?||No|
|GPS Coordinates||39.998871 N, 75.28797 W Map|
|Discovery Notes||Idergollasper was found under a tree at a corner of South Ardmore Park between Sussex Road and Athens Avenue in Wynnewood, Pennsylvania. The soil sample was collected under a tree located closer to the side of Sussex Road by Julia Iacovella on Monday, January 25, 2021 at 3:00 pm. The depth of the soil was 13 cm, and the soil was hard and dry. The weather conditions when the soil sample was collected was cold and windy with overcast. The ambient temperature was 4.44 degrees Celsius and the ambient humidity was 50%. |
The titer of the HTL is 1.7 x 10^9 pfu/mL. The concentration of Idergollasper's DNA is 203.0 ng/uL. The OD 260/280 ratio is 1.82.
The EM image shows that Idergollasper phage particles have a prolate capsid head and a non-contractile tail. Average Idergollasper phage particles measure 150 nm (capsid length), 25 nm (capsid width), and 275 nm (capsid tail), N=3.
Because Idergollasper's restriction digest is similar to other Cluster O's virtual digest, it is likely that Idergollasper belongs to Cluster O.
|Naming Notes||Idergollasper is the name of a random playlist that was suggested to me on Spotify. Idergollasper is also my study playlist, and it was playing when I was collecting one of my soil sample.|
|Genome length (bp)||Unknown|
|Character of genome ends||Unknown|
|Fasta file available?||No|
|Cluster Life Cycle||Lytic|
|Annotating Institution||Unknown or unassigned|
|Annotation Status||Not sequenced|
|Plaque Notes||Idergollasper forms small, clear plaques less than 1 mm in diameter with small, cloudy bumps surrounding each plaque. The plaques were formed after 24 to 72 hours in the incubator at 37 degrees Celsius.|
|Has been Phamerated?||No|
|Uploaded to GenBank?||No|
|GenBank Accession||None yet|
|Refseq Number||None yet|
|Archiving status||Not in Pitt Archives|
|Restriction Digest Picture||Download|