Mycobacterium phage FoulBall
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Detailed Information for Phage FoulBall
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByNicole Bowen
Year Found2023
Location FoundGlenside, PA United States
Finding InstitutionSaint Joseph's University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.099589 N, 75.142356 W Map
Discovery NotesPhage was collected on January 15th, 2023 at 4:15pm at Hallowell Park in Glenside, PA 19038. I dug in soil in a dried up creek bed that was about 50 feet from a baseball field and 150 feet from a fastener factory.
Soil condition- moist, saturated,sandy
Depth- 17.78 cm
Temperature- 0.6 C
Weather conditions- cold, cloudy, dry
Naming NotesWhen I was digging in the soil of the dried up creek bed to collect my sample, I dug up 2 baseballs.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJul 18, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1174
Genome length (bp)71074
Character of genome ends3' Sticky Overhang
Overhang Length4 bases
Overhang SequenceGTGT
GC Content65.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterO
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesLytic
Other Cluster Members
Annotating InstitutionSaint Joseph's University
Annotation StatusIn GenBank
Plaque Notesclear, 1mm plaques
Webbed plates exhibited 3/4 mm plaques
incubation length= 48 hours
Has been Phamerated?Yes
Gene List

Click to View

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Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP978769
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 166
Pitt Freezer Box Grid# E9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload