| Detailed Information for Phage Forrest |
| Discovery Information |
| Isolation Host | Streptomyces viridochromogenes DSM40736 |
| Found By | Vu Mather and Amy Chen |
| Year Found | 2015 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 24°C |
| GPS Coordinates | 38.646667 N, 90.310833 W Map |
| Discovery Notes | Found in the Elizabeth Danforth Butterfly Garden near some milkweed plants. The soil was loose and dry and sample depth was 4 cm. |
| Naming Notes | We found it in a forest/butterfly garden and we love Forrest Gump..... so why not? |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 29, 2020 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 93 |
| Genome length (bp) | 128513 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 766 bp |
| GC Content | 47.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BK |
| Subcluster | BK1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | After four rounds of purification, our plaques consolidated in morphology. They were all small in size, about 1 mm in diameter. Each plaque was also completely clear and had very distinct borders. |
| Morphotype | Siphoviridae |
| Number of Genes | 229 |
| Number of tRNAs | 37 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ820093 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
5.0x10^10 pfu/mL |
| Date of SEA Lysate Titering |
Oct 8, 2015 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
