| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Ihunanya Nwoji & Jessica Schultz |
| Year Found | 2018 |
| Location Found | Pasadena, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.140777 N, 76.528469 W Map |
| Discovery Notes | Isolated from soil collected 9/11/18 from the surface of a grassy hill. Soil was damp and sandy and collected on an overcast day with 93% humidity and an ambient temperature of 23 °C. |
| Naming Notes | The phage name is derived from the name of the compound geosmin, which is produced by streptomycetes and is responsible for the distinct scent of soil. The ending of the name comes from the beginning letters of Tick Neck Park, where the phage was found. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 24, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1661 |
| Genome length (bp) | 46175 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 253 bp |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are about 1.1 mm in diameter and have clear centers and turbid edges when grown at 28 °C for 24 hours on S. scabiei on NA+. |
| Morphotype | Podoviridae |
| Number of Genes | 66 |
| Number of tRNAs | 16 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN119376 |
| Refseq Number | None yet |
| Published in Paper | Davis et al 2022, Microbiology Resource Announcements: Genome Sequences of Streptomyces Bacteriophages Fabian, FlowerPower, Geostin, RetrieverFever, and Vorvolakos |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4x10^8 pfu/mL |
| Pitt Freezer Box# |
83 |
| Pitt Freezer Box Grid# |
F4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
